Total protein and acid phosphatase concentrations in prostatic fluid from patients with BPH compared to carcinoma

Edward Kim, N. D. Smith, J. T. Grayhack

Research output: Contribution to journalArticle

Abstract

Objective: To obtain evidence of metabolic changes in the human prostate associated with prostate pathology, in particular carcinoma of the prostate, by identifying and evaluating associated changes in prostatic secretory products. Methods: Expressed prostatic fluid (EPF) from 36 patients with carcinoma, 128 with BPH histologically confirmed, and 148 with clinical BPH was subjected to determination of protein (Lowry; UV 280 nm absorption), enzymatic (DMA modified Row procedure) acid phosphatase (AcP), and immunologically identified (Tandem®-PAP immunoenzymatic assay) prostatic acid phosphatase (PAP) concentration. Results: The important EPF findings are the following: (1) Protein concentrations (Lowry and UV determinations) are significantly increased in carcinoma as compared to histologic BPH, (2) AcP and PAP secretions remain stable in carcinoma versus BPH, and (3) ACP and PAP/Lowry protein ratios are significantly lower with carcinoma. Conclusions: These findings of increased protein and the decreased relative secretions of AcP and PAP to total protein (ratio) in EPF from patients with carcinoma compared to BPH support and help to characterize the diffuse metabolic alteration in the prostate associated with prostate carcinoma. EPF observations identify potential metabolic changes occurring in prostate carcinoma that may have potential clinical and investigative relevance.

Original languageEnglish (US)
Pages (from-to)471-479
Number of pages9
JournalInternational Urology and Nephrology
Volume30
Issue number4
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Phosphoprotein Phosphatases
Acid Phosphatase
Carcinoma
Prostate
Proteins
prostatic acid phosphatase
Pathology

All Science Journal Classification (ASJC) codes

  • Nephrology
  • Urology

Cite this

Total protein and acid phosphatase concentrations in prostatic fluid from patients with BPH compared to carcinoma. / Kim, Edward; Smith, N. D.; Grayhack, J. T.

In: International Urology and Nephrology, Vol. 30, No. 4, 01.01.1998, p. 471-479.

Research output: Contribution to journalArticle

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