Trace Measurements of RNA by Potentiometric Stripping Analysis at Carbon Paste Electrodes

Joseph Wang, Xiaohua Cai, Jianyan Wang, Colleen Jonsson, Emil Paleček

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Remarkably low levels of ribonucleic acid (RNA) can be measured by coupling its adsorptive accumulation onto carbon paste electrodes with constant current potentiometric stripping analysis (PSA). The computerized PSA operation effectively addresses the high background response inherent to carbon surfaces, while the anodic pretreatment of the electrode greatly enhances the preconcentration efficiency. The detection limit for tRNA (10 pg, 4 × 1016 mol) is substantially lower than that reported recently (Palaček, E.; Fojta, M. Anal, Chem. 1994, 66, 1566) for analogous voltammetric measurements at mercury surfaces. Variables influencing the accumulation and stripping processes of RNA are explored and optimized. Results are reported for measurements of RNA in the presence of excess dsDNA, for PSA of synthetic polyribonucleotides, for hybridization of complementaty strands of synthetic polyribonucleotides, for enzymatic hydrolysis of RNA, and for flow injection operation. Such solid electrode experiments obviate the need for mercury electrodes or a deoxygenation step and open the door for modem RNA detectors and probes.

Original languageEnglish (US)
Pages (from-to)4065-4070
Number of pages6
JournalAnalytical Chemistry
Volume67
Issue number22
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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Ointments
Carbon
RNA
Electrodes
Polyribonucleotides
Mercury
Enzymatic hydrolysis
Modems
Transfer RNA
Detectors
Experiments

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Trace Measurements of RNA by Potentiometric Stripping Analysis at Carbon Paste Electrodes. / Wang, Joseph; Cai, Xiaohua; Wang, Jianyan; Jonsson, Colleen; Paleček, Emil.

In: Analytical Chemistry, Vol. 67, No. 22, 01.01.1995, p. 4065-4070.

Research output: Contribution to journalArticle

Wang, Joseph ; Cai, Xiaohua ; Wang, Jianyan ; Jonsson, Colleen ; Paleček, Emil. / Trace Measurements of RNA by Potentiometric Stripping Analysis at Carbon Paste Electrodes. In: Analytical Chemistry. 1995 ; Vol. 67, No. 22. pp. 4065-4070.
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