Transcription of the Rous sarcoma virus genome in vitro and in vivo

J. M. Bishop, C. T. Deng, A. J. Faras, H. M. Goodman, Ramareddy Guntaka, W. E. Levinson, B. Cordell-Stewart, J. M. Taylor, H. E. Varmus

Research output: Contribution to journalArticle

Abstract

RNA directed DNA synthesis by detergent disrupted virions of Rous sarcoma virus (RSV) initiates by the covalent attachment of pdA to the 3' terminal rA of a 4S RNA hydrogen bonded to the 70S RNA template. This 4S 'primer' has structural features of tRNA and can be aminoacylated with methionine. Synthesis and integration of provirus DNA can be monitored in both permissive (duck) and nonpermissive (mouse) cells acutely infected with RSV. The results of these studies, as well as data obtained with RSV infected mammalian cells which have reverted from a transformed to a phenotypically normal state, indicate that integration of viral genes into the host chromosome is not sufficient cause for transformation. Pertinent features of virus specific RNA directed DNA synthesis in vitro and in vivo are reviewed and compared.

Original languageEnglish (US)
Pages (from-to)517-523
Number of pages7
JournalBibliotheca Haematologica
Volumeno.40
StatePublished - Dec 1 1975
Externally publishedYes

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Rous sarcoma virus
Genome
RNA
DNA
Virus Integration
Viral Genes
Ducks
RNA Viruses
Transfer RNA
Methionine
Virion
Detergents
Hydrogen
Chromosomes
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Bishop, J. M., Deng, C. T., Faras, A. J., Goodman, H. M., Guntaka, R., Levinson, W. E., ... Varmus, H. E. (1975). Transcription of the Rous sarcoma virus genome in vitro and in vivo. Bibliotheca Haematologica, no.40, 517-523.

Transcription of the Rous sarcoma virus genome in vitro and in vivo. / Bishop, J. M.; Deng, C. T.; Faras, A. J.; Goodman, H. M.; Guntaka, Ramareddy; Levinson, W. E.; Cordell-Stewart, B.; Taylor, J. M.; Varmus, H. E.

In: Bibliotheca Haematologica, Vol. no.40, 01.12.1975, p. 517-523.

Research output: Contribution to journalArticle

Bishop, JM, Deng, CT, Faras, AJ, Goodman, HM, Guntaka, R, Levinson, WE, Cordell-Stewart, B, Taylor, JM & Varmus, HE 1975, 'Transcription of the Rous sarcoma virus genome in vitro and in vivo', Bibliotheca Haematologica, vol. no.40, pp. 517-523.
Bishop JM, Deng CT, Faras AJ, Goodman HM, Guntaka R, Levinson WE et al. Transcription of the Rous sarcoma virus genome in vitro and in vivo. Bibliotheca Haematologica. 1975 Dec 1;no.40:517-523.
Bishop, J. M. ; Deng, C. T. ; Faras, A. J. ; Goodman, H. M. ; Guntaka, Ramareddy ; Levinson, W. E. ; Cordell-Stewart, B. ; Taylor, J. M. ; Varmus, H. E. / Transcription of the Rous sarcoma virus genome in vitro and in vivo. In: Bibliotheca Haematologica. 1975 ; Vol. no.40. pp. 517-523.
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