Transcriptional activity in lysates of cultured mesenchymal cells from embryonic tooth germs

Daniel Kestler, E. J. Kollar, E. F. Rossomando

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mesenchymal cells isolated from the papilla of embryonic tooth germs of the mouse were cultured in a complex medium for five to six days. Liquid nitrogen lysates, prepared from these cells, incorporated nucleoside monophosphates into a cold acid‐insoluble product. The product was sensitive to RNase and no product was formed if the lysate was pretreated with DNase. The reaction was sensitive to EDTA and, in its presence, optimum activity was obtained with 2 mM MgCl2. On sucrose gradients, the reaction product was distributed between two broad peaks; one centered about 18S and the other above 28S. The RNA polymerase inhibitor α‐amanitin inhibited approximately 50% of the activity at a concentration of 10 μg/ml.

Original languageEnglish (US)
Pages (from-to)109-112
Number of pages4
JournalJournal of Experimental Zoology
Volume212
Issue number1
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

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cultured cells
teeth
amanitins
deoxyribonucleases
nucleosides
DNA-directed RNA polymerase
sucrose
cells
liquids
mice
nitrogen

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Transcriptional activity in lysates of cultured mesenchymal cells from embryonic tooth germs. / Kestler, Daniel; Kollar, E. J.; Rossomando, E. F.

In: Journal of Experimental Zoology, Vol. 212, No. 1, 01.01.1980, p. 109-112.

Research output: Contribution to journalArticle

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