Transcriptional regulation of the DAL5 gene in Saccharomyces cerevisiae.

R. Rai, F. Genbauffe, H. Z. Lea, Terrance Cooper

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We demonstrate that the DAL5 gene, encoding a necessary component of the allantoate transport system, is constitutively expressed in Saccharomyces cerevisiae. Its relatively high basal level of expression did not increase further upon addition of allantoin pathway intermediates. However, steady-state DAL5 mRNA levels dropped precipitously when a repressive nitrogen source was provided. These control characteristics of DAL5 expression make this gene a good model with which to unravel the mechanism of nitrogen catabolite repression. Its particular advantage relative to other potentially useful genes derives from its lack of control by induction and hence the complicating effects of inducer exclusion.

Original languageEnglish (US)
Pages (from-to)3521-3524
Number of pages4
JournalJournal of Bacteriology
Volume169
Issue number8
DOIs
StatePublished - Jan 1 1987

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Saccharomyces cerevisiae
Nitrogen
Allantoin
Catabolite Repression
Genes
Gene Expression
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Transcriptional regulation of the DAL5 gene in Saccharomyces cerevisiae. / Rai, R.; Genbauffe, F.; Lea, H. Z.; Cooper, Terrance.

In: Journal of Bacteriology, Vol. 169, No. 8, 01.01.1987, p. 3521-3524.

Research output: Contribution to journalArticle

Rai, R. ; Genbauffe, F. ; Lea, H. Z. ; Cooper, Terrance. / Transcriptional regulation of the DAL5 gene in Saccharomyces cerevisiae. In: Journal of Bacteriology. 1987 ; Vol. 169, No. 8. pp. 3521-3524.
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