Transcriptomic analysis on the formation of the viable putative non-culturable state of beer-spoilage Lactobacillus acetotolerans

Junyan Liu, Yang Deng, Brian Peters, Lin Li, Bing Li, Lequn Chen, Zhenbo Xu, Mark E. Shirtliff

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Lactic acid bacteria (LAB) are the most common beer-spoilage bacteria regardless of beer type, and thus pose significant problems for the brewery industry. The aim of this study was to investigate the genetic mechanisms involved in the ability of the hard-to-culture beer-spoilage bacterium Lactobacillus acetotolerans to enter into the viable putative non-culturable (VPNC) state. A genome-wide transcriptional analysis of beer-spoilage L. acetotolerans strains BM-LA14526, BM-LA14527, and BM-LA14528 under normal, mid-term and VPNC states were performed using RNA-sequencing (RNA-seq) and further bioinformatics analyses. GO function, COG category, and KEGG pathway enrichment analysis were conducted to investigate functional and related metabolic pathways of the differentially expressed genes. Functional and pathway enrichment analysis indicated that heightened stress response and reduction in genes associated with transport, metabolic process, and enzyme activity might play important roles in the formation of the VPNC state. This is the first transcriptomic analysis on the formation of the VPNC state of beer spoilage L. acetotolerans.

Original languageEnglish (US)
Article number36753
JournalScientific Reports
Volume6
DOIs
StatePublished - Nov 7 2016

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Lactobacillus
Bacteria
RNA Sequence Analysis
Metabolic Networks and Pathways
Computational Biology
Genes
Lactic Acid
Industry
Genome
RNA
Enzymes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Transcriptomic analysis on the formation of the viable putative non-culturable state of beer-spoilage Lactobacillus acetotolerans. / Liu, Junyan; Deng, Yang; Peters, Brian; Li, Lin; Li, Bing; Chen, Lequn; Xu, Zhenbo; Shirtliff, Mark E.

In: Scientific Reports, Vol. 6, 36753, 07.11.2016.

Research output: Contribution to journalArticle

Liu, Junyan ; Deng, Yang ; Peters, Brian ; Li, Lin ; Li, Bing ; Chen, Lequn ; Xu, Zhenbo ; Shirtliff, Mark E. / Transcriptomic analysis on the formation of the viable putative non-culturable state of beer-spoilage Lactobacillus acetotolerans. In: Scientific Reports. 2016 ; Vol. 6.
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