Transforming growth factor-β effects on meningioma cell proliferation and signal transduction pathways

Mahlon Johnson, Evelyn Okediji, Ann Woodard

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31 Citations (Scopus)

Abstract

The role of transforming growth factor-β (TGF-β) in regulation of meningioma growth and intracellular events transducing its signals are not established. In this study, we evaluated the effects of TGF-β1 on basal meningioma cell proliferation in 10 primary human meningioma cell cultures and whether TGF-β's signals are transduced by the Smad 2/3, MAPK/Erk kinase-1(MEK-1)-mitogen-activated protein kinase (MAPK), Akt-p70S6K or p38-JUNK pathways in 5. We also tested whether neutralizing antibodies to TGF-β alter CSF stimulation of meningioma cell proliferation. On average, TGF-β reduced meningioma cell [3H]-thymidine incorporation to 58% of controls at 24% and to 61% of controls at 36 h. TGF-β inhibition of meningioma cell proliferation was associated with a suggestion increased phosphorylation of Smad 2/3 in 2 cases and high basal phosphorylation in 3 but no change in activation of the MEK-1-MAPK, Akt-p70S6K or p38-JUNK pathways. As shown previously, CSF stimulated meningioma cell proliferation in the 3 cultures tested. Neutralizing antibody against TGF-β augmented this stimulation in 2 of 3 cultures. These findings suggest that TGF-β exerts a largely inhibitory effect on basal meningioma proliferation, perhaps in part through Smad 2/3.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalJournal of Neuro-Oncology
Volume66
Issue number1-2
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

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Transforming Growth Factors
Meningioma
Signal Transduction
Cell Proliferation
MAP Kinase Kinase 1
70-kDa Ribosomal Protein S6 Kinases
Mitogen-Activated Protein Kinases
Neutralizing Antibodies
Phosphorylation
Thymidine
Cell Culture Techniques
Growth

All Science Journal Classification (ASJC) codes

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

Cite this

Transforming growth factor-β effects on meningioma cell proliferation and signal transduction pathways. / Johnson, Mahlon; Okediji, Evelyn; Woodard, Ann.

In: Journal of Neuro-Oncology, Vol. 66, No. 1-2, 01.01.2004, p. 9-16.

Research output: Contribution to journalArticle

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