Transforming growth factor β increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts

Rajendra Raghow, Arnold Postlethwaite, J. Keski-Oja, H. L. Moses, Andrew Kang

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340 Citations (Scopus)

Abstract

Transforming growth factor-β (TGFβ), when injected subcutaneously into newborn mice, induces a rapid fibrotic response, stimulates chemotaxis, and elevates the rates of biosynthesis of collagen and fibronectin by fibroblasts in vitro. We explored the molecular mechanisms of TGFβ-mediated stimulation of collagen and fibronectin synthesis in cultured human foreskin fibroblasts. TGFβ preferentially stimulated the synthesis of fibronectin and type I procollagen chains 3-5-fold as shown by polypeptide analysis. Concomitant elevation in the steady state levels of messenger RNAs (mRNAs) coding for type I procollagen and fibronectin also occurred but without a net increase in the rate of transcription of either of these genes. The preferential stabilization of mRNAs specifying type I procollagen and fibronectin provides a partial explanation for the mechanisms by which TGFβ enhances the synthesis of type I procollagen and fibronectin in mesenchymal cells.

Original languageEnglish (US)
Pages (from-to)1285-1288
Number of pages4
JournalJournal of Clinical Investigation
Volume79
Issue number4
DOIs
StatePublished - Jan 1 1987

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Transforming Growth Factors
Collagen Type I
Fibronectins
Fibroblasts
Messenger RNA
Skin
Collagen
Foreskin
Chemotaxis
Peptides
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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T1 - Transforming growth factor β increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts

AU - Raghow, Rajendra

AU - Postlethwaite, Arnold

AU - Keski-Oja, J.

AU - Moses, H. L.

AU - Kang, Andrew

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Transforming growth factor-β (TGFβ), when injected subcutaneously into newborn mice, induces a rapid fibrotic response, stimulates chemotaxis, and elevates the rates of biosynthesis of collagen and fibronectin by fibroblasts in vitro. We explored the molecular mechanisms of TGFβ-mediated stimulation of collagen and fibronectin synthesis in cultured human foreskin fibroblasts. TGFβ preferentially stimulated the synthesis of fibronectin and type I procollagen chains 3-5-fold as shown by polypeptide analysis. Concomitant elevation in the steady state levels of messenger RNAs (mRNAs) coding for type I procollagen and fibronectin also occurred but without a net increase in the rate of transcription of either of these genes. The preferential stabilization of mRNAs specifying type I procollagen and fibronectin provides a partial explanation for the mechanisms by which TGFβ enhances the synthesis of type I procollagen and fibronectin in mesenchymal cells.

AB - Transforming growth factor-β (TGFβ), when injected subcutaneously into newborn mice, induces a rapid fibrotic response, stimulates chemotaxis, and elevates the rates of biosynthesis of collagen and fibronectin by fibroblasts in vitro. We explored the molecular mechanisms of TGFβ-mediated stimulation of collagen and fibronectin synthesis in cultured human foreskin fibroblasts. TGFβ preferentially stimulated the synthesis of fibronectin and type I procollagen chains 3-5-fold as shown by polypeptide analysis. Concomitant elevation in the steady state levels of messenger RNAs (mRNAs) coding for type I procollagen and fibronectin also occurred but without a net increase in the rate of transcription of either of these genes. The preferential stabilization of mRNAs specifying type I procollagen and fibronectin provides a partial explanation for the mechanisms by which TGFβ enhances the synthesis of type I procollagen and fibronectin in mesenchymal cells.

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