Transforming growth factor β1 gene ablation leads to altered rates of cell proliferation and extracellular matrix biosynthesis

C. Sudarshan, L. Yaswen, A. Kulkarni, Rajendra Raghow

Research output: Contribution to journalArticle

Abstract

Transforming growth factor β1 null (TGF β1-/-) mice have been invaluable in elucidating the varied functions of this cytokine. A subset of TGF β1 null embryos die in utero due to developmental deformities; functionally redundant congeners of TGF β1 (e.g. TGF βor TGF β3) and maternal transfer of TGF β1 rescue a fraction of TGF β1-/- embryos, which die perinatally from dysfunctional immune response. These elegant observations notwithstanding, it has been difficult to assess the precise autocrine versus paracrine effects of TGF β1 on individual cell lineages in the intact organism. To circumvent this, we examined the rates of cell proliferation and extracellular matrix (ECM) phenotype of embryonic fibroblasts (MEF 14 KO) derived from TGF β1-/- mice. MEF 14 KO cells multiplied at a higher rate than their normal counterparts, as judged by cell counting, 3 H-thymidine incorporation and F ACS analysis. The steady state levels of mRNAs encoding Proal(I) collagen, fibronectin and plasminogen activator inhibitor-1 were also significantly lower in MEF 14 KO cells. MEF 14 KO cells expressed all three types of TGF β1 receptors and responded normally to exogenous TGF β1. Based on these data we suggest that TGF β1 is an autocrine regulator of growth and ECM biosynthesis in embryonic fibroblasts.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

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Biosynthesis
Cell proliferation
Transforming Growth Factors
Ablation
Extracellular Matrix
Genes
Cell Proliferation
Fibroblasts
Embryonic Structures
Plasminogen Activator Inhibitor 1
Set theory
Fibronectins
Thymidine
Cell Lineage
Collagen
Cytokines
Messenger RNA
Mothers
Phenotype
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Transforming growth factor β1 gene ablation leads to altered rates of cell proliferation and extracellular matrix biosynthesis. / Sudarshan, C.; Yaswen, L.; Kulkarni, A.; Raghow, Rajendra.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

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