Transmissibility of systemic amyloidosis by a prion-like mechanism

Katarzyna Lundmark, Gunilla T. Westermark, Sofia Nyström, Charles L. Murphy, Alan Solomon, Per Westermark

Research output: Contribution to journalArticle

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Abstract

The generation of amyloid fibrils from an amyloidogenic polypeptide occurs by a nucleation-dependent process initiated in vitro by seeding the protein solution with preformed fibrils. This phenomenon is evidenced in vivo by the fact that amyloid protein A (AA) amyloidosis in mice is markedly accelerated when the animals are given, in addition to an inflammatory stimulus, an i.v. injection of protein extracted from AA amyloid-laden mouse tissue. Heretofore, the chemical nature of this "amyloid enhancing factor" (AEF) has not been definitively identified. Here we report that the active principle of AEF extracted from the spleen of mice with silver nitrate-induced AA amyloidosis was identified unequivocally as the AA fibril itself. Further, we demonstrated that this material was extremely potent, being active in doses <1 ng, and that it retained its biologic activity over a considerable length of time. Notably, the AEF was also effective when administered orally. Our studies have provided evidence that AA and perhaps other forms of amyloidosis are transmissible diseases, akin to the prion-associated disorders.

Original languageEnglish (US)
Pages (from-to)6979-6984
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number10
DOIs
StatePublished - May 14 2002

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Serum Amyloid A Protein
Prions
Amyloidosis
Amyloid
Silver Nitrate
Prion Diseases
Proteins
Spleen
Peptides
Injections
amyloid enhancing factor

All Science Journal Classification (ASJC) codes

  • General

Cite this

Transmissibility of systemic amyloidosis by a prion-like mechanism. / Lundmark, Katarzyna; Westermark, Gunilla T.; Nyström, Sofia; Murphy, Charles L.; Solomon, Alan; Westermark, Per.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 10, 14.05.2002, p. 6979-6984.

Research output: Contribution to journalArticle

Lundmark, Katarzyna ; Westermark, Gunilla T. ; Nyström, Sofia ; Murphy, Charles L. ; Solomon, Alan ; Westermark, Per. / Transmissibility of systemic amyloidosis by a prion-like mechanism. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 10. pp. 6979-6984.
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