Transmission of (LTR, v-src, LTR) without recombination with a helper virus

Jan Svoboda, Michal Dvořák, Ramareddy Guntaka, Josef Geryk

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have analyzed transmission of (LTR, v-src, LTR) cryptic structure integrated in the H-19 mammalian tumor cell line (1). From this cell line different isolates of transforming virus were rescued in heterokaryons produced by fusion with chicken fibroblasts infected by replication-competent avian leukosis virus RAV-1 (2). One of them (F6) was used for the transformation of avian cells in the absence of the helper virus. In four transformed cell lines studied, the (LTR, v-src, LTR) structure was again integrated at a unique position in the cell DNA of each line. This indicated that the (LTR, v-src, LTR) structure is transmitted by the helper virus without recombination. This point has been further supported by the finding that a src-containing species corresponding in size to the nonpolyadenylated src mRNA is present in the RNA isolated from the rescued F6 transforming virus which might serve as template for the synthesis of (LTR, v-src, LTR) structure by the reverse transcriptase provided by RAV-1.

Original languageEnglish (US)
Pages (from-to)314-317
Number of pages4
JournalVirology
Volume153
Issue number2
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Helper Viruses
Genetic Recombination
Avian Leukosis Virus
Viruses
Transformed Cell Line
RNA-Directed DNA Polymerase
Tumor Cell Line
Chickens
Fibroblasts
RNA
Cell Line
Messenger RNA
DNA

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Transmission of (LTR, v-src, LTR) without recombination with a helper virus. / Svoboda, Jan; Dvořák, Michal; Guntaka, Ramareddy; Geryk, Josef.

In: Virology, Vol. 153, No. 2, 01.01.1986, p. 314-317.

Research output: Contribution to journalArticle

Svoboda, Jan ; Dvořák, Michal ; Guntaka, Ramareddy ; Geryk, Josef. / Transmission of (LTR, v-src, LTR) without recombination with a helper virus. In: Virology. 1986 ; Vol. 153, No. 2. pp. 314-317.
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