Tripartite structure of the Saccharomyces cerevisiae arginase (CAR1) gene inducer-responsive upstream activation sequence

M. Viljoen, L. Z. Kovari, I. A. Kovari, H. D. Park, H. J.J. Van Vuuren, Terrance Cooper

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Arginase (CAR1) gene expression in Saccharomyces cerevisiae is induced by arginine. The 5' regulatory region of CAR1 contains four separable regulatory elements-two inducer-independent upstream activation sequences (UASs) (UAS(C1) and UAS(C2)), an inducer-dependent UAS (UAS(I)), and an upstream repression sequence (URS1) which negatively regulates CAR1 and many other yeast genes. Here we demonstrate that three homologous DNA sequences originally reported to be present in the inducer-responsive UAS(I) are in fact three exchangeable elements (UAS(I-A), UAS(I-B), and UAS(I-C)). Although two of these elements, either the same or different ones, are required for transcriptional activation to occur, all three are required for maximal levels of induction. The elements operate in all orientations relative to one another and to the TATA sequence. All three UAS(I) elements bind protein(s); protein binding does not require arginine or overproduction of any of the putative arginine pathway regulatory proteins. The UAS(I)-protein complex was also observed even when extracts were derived from arg80/argRI or arg81/argRII deletion mutants. Similar sequences situated upstream of ARG5,6 and ARG3 and reported to negatively regulate their expression are able to functionally substitute for the CAR1 UAS(I) elements and mediate reporter gene expression.

Original languageEnglish (US)
Pages (from-to)6831-6839
Number of pages9
JournalJournal of Bacteriology
Volume174
Issue number21
DOIs
StatePublished - Jan 1 1992

Fingerprint

Arginase
Saccharomyces cerevisiae
Arginine
Genes
Gene Expression
Proteins
Nucleic Acid Regulatory Sequences
Sequence Homology
Reporter Genes
Protein Binding
Transcriptional Activation
Yeasts

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Tripartite structure of the Saccharomyces cerevisiae arginase (CAR1) gene inducer-responsive upstream activation sequence. / Viljoen, M.; Kovari, L. Z.; Kovari, I. A.; Park, H. D.; Van Vuuren, H. J.J.; Cooper, Terrance.

In: Journal of Bacteriology, Vol. 174, No. 21, 01.01.1992, p. 6831-6839.

Research output: Contribution to journalArticle

Viljoen, M. ; Kovari, L. Z. ; Kovari, I. A. ; Park, H. D. ; Van Vuuren, H. J.J. ; Cooper, Terrance. / Tripartite structure of the Saccharomyces cerevisiae arginase (CAR1) gene inducer-responsive upstream activation sequence. In: Journal of Bacteriology. 1992 ; Vol. 174, No. 21. pp. 6831-6839.
@article{accfef59b92c45b48ab04c55c659cf6c,
title = "Tripartite structure of the Saccharomyces cerevisiae arginase (CAR1) gene inducer-responsive upstream activation sequence",
abstract = "Arginase (CAR1) gene expression in Saccharomyces cerevisiae is induced by arginine. The 5' regulatory region of CAR1 contains four separable regulatory elements-two inducer-independent upstream activation sequences (UASs) (UAS(C1) and UAS(C2)), an inducer-dependent UAS (UAS(I)), and an upstream repression sequence (URS1) which negatively regulates CAR1 and many other yeast genes. Here we demonstrate that three homologous DNA sequences originally reported to be present in the inducer-responsive UAS(I) are in fact three exchangeable elements (UAS(I-A), UAS(I-B), and UAS(I-C)). Although two of these elements, either the same or different ones, are required for transcriptional activation to occur, all three are required for maximal levels of induction. The elements operate in all orientations relative to one another and to the TATA sequence. All three UAS(I) elements bind protein(s); protein binding does not require arginine or overproduction of any of the putative arginine pathway regulatory proteins. The UAS(I)-protein complex was also observed even when extracts were derived from arg80/argRI or arg81/argRII deletion mutants. Similar sequences situated upstream of ARG5,6 and ARG3 and reported to negatively regulate their expression are able to functionally substitute for the CAR1 UAS(I) elements and mediate reporter gene expression.",
author = "M. Viljoen and Kovari, {L. Z.} and Kovari, {I. A.} and Park, {H. D.} and {Van Vuuren}, {H. J.J.} and Terrance Cooper",
year = "1992",
month = "1",
day = "1",
doi = "10.1128/jb.174.21.6831-6839.1992",
language = "English (US)",
volume = "174",
pages = "6831--6839",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "21",

}

TY - JOUR

T1 - Tripartite structure of the Saccharomyces cerevisiae arginase (CAR1) gene inducer-responsive upstream activation sequence

AU - Viljoen, M.

AU - Kovari, L. Z.

AU - Kovari, I. A.

AU - Park, H. D.

AU - Van Vuuren, H. J.J.

AU - Cooper, Terrance

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Arginase (CAR1) gene expression in Saccharomyces cerevisiae is induced by arginine. The 5' regulatory region of CAR1 contains four separable regulatory elements-two inducer-independent upstream activation sequences (UASs) (UAS(C1) and UAS(C2)), an inducer-dependent UAS (UAS(I)), and an upstream repression sequence (URS1) which negatively regulates CAR1 and many other yeast genes. Here we demonstrate that three homologous DNA sequences originally reported to be present in the inducer-responsive UAS(I) are in fact three exchangeable elements (UAS(I-A), UAS(I-B), and UAS(I-C)). Although two of these elements, either the same or different ones, are required for transcriptional activation to occur, all three are required for maximal levels of induction. The elements operate in all orientations relative to one another and to the TATA sequence. All three UAS(I) elements bind protein(s); protein binding does not require arginine or overproduction of any of the putative arginine pathway regulatory proteins. The UAS(I)-protein complex was also observed even when extracts were derived from arg80/argRI or arg81/argRII deletion mutants. Similar sequences situated upstream of ARG5,6 and ARG3 and reported to negatively regulate their expression are able to functionally substitute for the CAR1 UAS(I) elements and mediate reporter gene expression.

AB - Arginase (CAR1) gene expression in Saccharomyces cerevisiae is induced by arginine. The 5' regulatory region of CAR1 contains four separable regulatory elements-two inducer-independent upstream activation sequences (UASs) (UAS(C1) and UAS(C2)), an inducer-dependent UAS (UAS(I)), and an upstream repression sequence (URS1) which negatively regulates CAR1 and many other yeast genes. Here we demonstrate that three homologous DNA sequences originally reported to be present in the inducer-responsive UAS(I) are in fact three exchangeable elements (UAS(I-A), UAS(I-B), and UAS(I-C)). Although two of these elements, either the same or different ones, are required for transcriptional activation to occur, all three are required for maximal levels of induction. The elements operate in all orientations relative to one another and to the TATA sequence. All three UAS(I) elements bind protein(s); protein binding does not require arginine or overproduction of any of the putative arginine pathway regulatory proteins. The UAS(I)-protein complex was also observed even when extracts were derived from arg80/argRI or arg81/argRII deletion mutants. Similar sequences situated upstream of ARG5,6 and ARG3 and reported to negatively regulate their expression are able to functionally substitute for the CAR1 UAS(I) elements and mediate reporter gene expression.

UR - http://www.scopus.com/inward/record.url?scp=0026675753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026675753&partnerID=8YFLogxK

U2 - 10.1128/jb.174.21.6831-6839.1992

DO - 10.1128/jb.174.21.6831-6839.1992

M3 - Article

VL - 174

SP - 6831

EP - 6839

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 21

ER -