Triplex-forming oligonucleotides as modulators of gene expression

Ramareddy Guntaka, Balwantkumar R. Varma, Karl Weber

Research output: Contribution to journalReview article

72 Citations (Scopus)

Abstract

Triplex-forming oligonucleotides (TFOs) have gained prominence in the recent years because of their potential applications in antigene therapy. In particular they have been used as (i) inducers of site-specific mutations, (ii) reagents that selectively and specifically cleave target DNA, and (iii) as modulators of gene expression. In this mini-review, we have made an attempt to highlight the characteristics of these TFOs and the effects of various modifications in the phosphate backbone as well as in the purine and pyrimidine moieties, which contribute to the stability and efficiency of triplex formation. Studies to explore the mechanism of down-regulation of transcription of various genes suggest that at least some TFOs exert their effect by inhibiting binding of specific transcription factors to their cognate cis-acting elements. Recent reports indicate the presence of these potential triplex-forming DNA structures in the genomes of prokaryotes and eukaryotes that may play a major role in target site selection and chromosome segregation as well as in the cause of heritable diseases. Finally, some potential problems in the development of these TFOs as antigene therapeutic agents have also been discussed.

Original languageEnglish (US)
Pages (from-to)22-31
Number of pages10
JournalInternational Journal of Biochemistry and Cell Biology
Volume35
Issue number1
DOIs
StatePublished - Jan 1 2003

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Gene expression
Oligonucleotides
Modulators
Gene Expression
Genes
Site selection
Chromosome Segregation
DNA
Transcription
Chromosomes
Eukaryota
Transcription Factors
Down-Regulation
Phosphates
Genome
Mutation
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology

Cite this

Triplex-forming oligonucleotides as modulators of gene expression. / Guntaka, Ramareddy; Varma, Balwantkumar R.; Weber, Karl.

In: International Journal of Biochemistry and Cell Biology, Vol. 35, No. 1, 01.01.2003, p. 22-31.

Research output: Contribution to journalReview article

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