Type 2 ryanodine receptors are highly sensitive to alcohol

Yanping Ye, Kuihuan Jian, Jonathan Jaggar, Anna Bukiya, Alejandro Dopico

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Exposure to ethanol levels reached in circulation during alcohol intoxication (>10 mM) constricts cerebral arteries in rats and humans. Remarkably, targets and mechanisms underlying this action remain largely unidentified. Artery diameter is regulated by myocyte Ca 2+ sparks, a vasodilatory signal contributed to by type 2 ryanodine receptors (RyR2). Using laser confocal microscopy in rat cerebral arteries and bilayer electrophysiology we unveil that ethanol inhibits both Ca 2+ spark and RyR2 activity with IC 50 < 20 mM, placing RyR2 among the ion channels that are most sensitive to ethanol. Alcohol directly targets RyR2 and its lipid microenvironment, leading to stabilization of RyR2 closed states.

Original languageEnglish (US)
Pages (from-to)1659-1665
Number of pages7
JournalFEBS Letters
Volume588
Issue number9
DOIs
StatePublished - May 2 2014

Fingerprint

Ryanodine Receptor Calcium Release Channel
Alcohols
Ethanol
Cerebral Arteries
Electric sparks
Confocal Microscopy
Rats
Electrophysiology
Alcoholic Intoxication
Confocal microscopy
Ion Channels
Muscle Cells
Stabilization
Arteries
Lipids
Lasers

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Type 2 ryanodine receptors are highly sensitive to alcohol. / Ye, Yanping; Jian, Kuihuan; Jaggar, Jonathan; Bukiya, Anna; Dopico, Alejandro.

In: FEBS Letters, Vol. 588, No. 9, 02.05.2014, p. 1659-1665.

Research output: Contribution to journalArticle

@article{d904f0f6869a47eaa522f4189ce70f03,
title = "Type 2 ryanodine receptors are highly sensitive to alcohol",
abstract = "Exposure to ethanol levels reached in circulation during alcohol intoxication (>10 mM) constricts cerebral arteries in rats and humans. Remarkably, targets and mechanisms underlying this action remain largely unidentified. Artery diameter is regulated by myocyte Ca 2+ sparks, a vasodilatory signal contributed to by type 2 ryanodine receptors (RyR2). Using laser confocal microscopy in rat cerebral arteries and bilayer electrophysiology we unveil that ethanol inhibits both Ca 2+ spark and RyR2 activity with IC 50 < 20 mM, placing RyR2 among the ion channels that are most sensitive to ethanol. Alcohol directly targets RyR2 and its lipid microenvironment, leading to stabilization of RyR2 closed states.",
author = "Yanping Ye and Kuihuan Jian and Jonathan Jaggar and Anna Bukiya and Alejandro Dopico",
year = "2014",
month = "5",
day = "2",
doi = "10.1016/j.febslet.2014.03.005",
language = "English (US)",
volume = "588",
pages = "1659--1665",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "9",

}

TY - JOUR

T1 - Type 2 ryanodine receptors are highly sensitive to alcohol

AU - Ye, Yanping

AU - Jian, Kuihuan

AU - Jaggar, Jonathan

AU - Bukiya, Anna

AU - Dopico, Alejandro

PY - 2014/5/2

Y1 - 2014/5/2

N2 - Exposure to ethanol levels reached in circulation during alcohol intoxication (>10 mM) constricts cerebral arteries in rats and humans. Remarkably, targets and mechanisms underlying this action remain largely unidentified. Artery diameter is regulated by myocyte Ca 2+ sparks, a vasodilatory signal contributed to by type 2 ryanodine receptors (RyR2). Using laser confocal microscopy in rat cerebral arteries and bilayer electrophysiology we unveil that ethanol inhibits both Ca 2+ spark and RyR2 activity with IC 50 < 20 mM, placing RyR2 among the ion channels that are most sensitive to ethanol. Alcohol directly targets RyR2 and its lipid microenvironment, leading to stabilization of RyR2 closed states.

AB - Exposure to ethanol levels reached in circulation during alcohol intoxication (>10 mM) constricts cerebral arteries in rats and humans. Remarkably, targets and mechanisms underlying this action remain largely unidentified. Artery diameter is regulated by myocyte Ca 2+ sparks, a vasodilatory signal contributed to by type 2 ryanodine receptors (RyR2). Using laser confocal microscopy in rat cerebral arteries and bilayer electrophysiology we unveil that ethanol inhibits both Ca 2+ spark and RyR2 activity with IC 50 < 20 mM, placing RyR2 among the ion channels that are most sensitive to ethanol. Alcohol directly targets RyR2 and its lipid microenvironment, leading to stabilization of RyR2 closed states.

UR - http://www.scopus.com/inward/record.url?scp=84899653578&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899653578&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2014.03.005

DO - 10.1016/j.febslet.2014.03.005

M3 - Article

VL - 588

SP - 1659

EP - 1665

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 9

ER -