Type IV Collagens

Isolation and Characterization of 7S Collagen from Human Kidney, Liver and Lung

S. N. Dixit, John Stuart, J. M. Seyer, J. Risteli, R. Timpl, Andrew Kang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

7S collagens were isolated after bacterial collagenase treatment of basement membrane material prepared from the pepsin digest of human kidney, liver and lung. The 7S collagens were purified by combination of molecular sieve and ion exchange chromatography. 7S collagen from each of these sources showed similar amino acid composition, electrophoretic patterns of reduced and unreduced samples. Antibodies raised against human placental 7S collagen in rabbits completely cross-reacted with the 7S collagen preparation obtained from kidney, liver and lung in enzyme-immunoassays. Since 7S collagen is known to be a major cross-linked segment of type IV collagens, the data indicate that collagenous proteins of basement membranes arc organized in similar networks in a variety of organs.

Original languageEnglish (US)
Pages (from-to)549-556
Number of pages8
JournalTopics in Catalysis
Volume1
Issue number6
DOIs
StatePublished - Jan 1 1981

Fingerprint

Collagen Type IV
Collagen
Liver
Kidney
Lung
Basement Membrane
Pepsin A
Molecular sieves
Ion Exchange Chromatography
Collagenases
Chromatography
Immunoenzyme Techniques
Antibodies
Amino acids
Ion exchange
Enzymes
Rabbits
Proteins
Amino Acids
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Rheumatology

Cite this

Type IV Collagens : Isolation and Characterization of 7S Collagen from Human Kidney, Liver and Lung. / Dixit, S. N.; Stuart, John; Seyer, J. M.; Risteli, J.; Timpl, R.; Kang, Andrew.

In: Topics in Catalysis, Vol. 1, No. 6, 01.01.1981, p. 549-556.

Research output: Contribution to journalArticle

Dixit, S. N. ; Stuart, John ; Seyer, J. M. ; Risteli, J. ; Timpl, R. ; Kang, Andrew. / Type IV Collagens : Isolation and Characterization of 7S Collagen from Human Kidney, Liver and Lung. In: Topics in Catalysis. 1981 ; Vol. 1, No. 6. pp. 549-556.
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