Type-specific collagenolysis: A type V collagen-degrading enzyme from macrophages

Carlo L. Mainardi, Jerome M. Seyer, Andrew Kang

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

An enzymatic activity capable of degrading type V collagen at neutral pH was found in the medium from cultured rabbit pulmonary alveolar macrophages which had been "activated" in vivo by injection of complete Freund's Adjuvant. This enzyme was characterized as a metalloproteinase by virtue of its inhibition by EDTA but not by phenylmethylsulfonyl fluoride or N-ethyl maleimide. Ion-exchange chromatography on DEAE-cellulose was successful in separating the type V collagen-degrading activity from the type I collagenase which is also secreted by these cells. These observations suggest that the degradation of type V collagen is independent of the degradation of the interstitial collagens and may require the action of its own "specific collagenase".

Original languageEnglish (US)
Pages (from-to)1108-1115
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume97
Issue number3
DOIs
StatePublished - Dec 16 1980

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Collagen Type V
Macrophages
Alveolar Macrophages
Collagenases
Phenylmethylsulfonyl Fluoride
Degradation
DEAE-Cellulose
Freund's Adjuvant
Ion Exchange Chromatography
Metalloproteases
Chromatography
Edetic Acid
Ion exchange
Collagen
Rabbits
Injections
Enzymes
type V collagen-degrading enzyme

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Type-specific collagenolysis : A type V collagen-degrading enzyme from macrophages. / Mainardi, Carlo L.; Seyer, Jerome M.; Kang, Andrew.

In: Biochemical and Biophysical Research Communications, Vol. 97, No. 3, 16.12.1980, p. 1108-1115.

Research output: Contribution to journalArticle

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