Tyrosine phosphorylation and dissociation of occludin-ZO-1 and E-cadherin-β-catenin complexes from the cytoskeleton by oxidative stress

Radhakrishna Rao, Shyamali Basuroy, Vijay U. Rao, Karl J. Karnaky, Akshay Gupta

Research output: Contribution to journalArticle

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Abstract

The oxidative-stress-induced alteration in paracellular junctional complexes was analysed in Caco-2 cell monolayer. Oxidative stress induced a rapid increase in tyrosine phosphorylation of occludin, zonula occludens (ZO)-1, E-cadherin and β-catenin. An oxidative-stress-induced decrease in transepithelial electrical resistance was associated with a redistribution of occludin-ZO-1 and E-cadherin-β-catenin complexes from the intercellular junctions. Genistein, a tyrosine kinase inhibitor, prevented the oxidative-stress-induced decrease in resistance and redistribution of protein complexes. Occludin, ZO-1, E-cadherin and β-catenin in the Triton-insoluble cytoskeletal fraction were reduced by oxidative stress, which was prevented by genistein. Oxidative stress also reduced the co-immunoprecipitation of ZO-1 with occludin, which was prevented by genistein. Co-immunoprecipitation of β-catenin with E-cadherin was unaffected by oxidative stress or genistein. ZO-1, E-cadherin and β-catenin in the plasma membrane or membrane-cytoskeleton were either slightly reduced or unaffected by oxidative stress or genistein. These results show that oxidative stress induces tyrosine phosphorylation and cellular redistribution of occludin-ZO-1 and E-cadherin-β-catenin complexes by a tyrosine-kinase-dependent mechanism.

Original languageEnglish (US)
Pages (from-to)471-481
Number of pages11
JournalBiochemical Journal
Volume368
Issue number2
DOIs
StatePublished - Dec 1 2002

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Occludin
Catenins
Phosphorylation
Oxidative stress
Tight Junctions
Cadherins
Cytoskeleton
Tyrosine
Oxidative Stress
Genistein
Immunoprecipitation
Protein-Tyrosine Kinases
Acoustic impedance
Intercellular Junctions
Caco-2 Cells
Cell membranes
Electric Impedance
Monolayers
Cell Membrane
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Tyrosine phosphorylation and dissociation of occludin-ZO-1 and E-cadherin-β-catenin complexes from the cytoskeleton by oxidative stress. / Rao, Radhakrishna; Basuroy, Shyamali; Rao, Vijay U.; Karnaky, Karl J.; Gupta, Akshay.

In: Biochemical Journal, Vol. 368, No. 2, 01.12.2002, p. 471-481.

Research output: Contribution to journalArticle

Rao, Radhakrishna ; Basuroy, Shyamali ; Rao, Vijay U. ; Karnaky, Karl J. ; Gupta, Akshay. / Tyrosine phosphorylation and dissociation of occludin-ZO-1 and E-cadherin-β-catenin complexes from the cytoskeleton by oxidative stress. In: Biochemical Journal. 2002 ; Vol. 368, No. 2. pp. 471-481.
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