Ultra-structural changes at the apical stop irradiated with CO2 laser

João Gonçalves Junior, Melissa Marchesan, Aline Evangelista Souza-Gabriel, Luis Pascoal Vansan, Manoel D. Sousa-Neto, Yara T.C. Silva-Sousa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: This study evaluated ultra-structural dentine changes at the apical stop after CO2 laser irradiation used during biomechanical preparation. Background: Most studies evaluating the sealing efficiency of CO2 lasers have been carried out after apical root canal resections and retro-filling procedures. Methods: Sixty human canines were prepared with #1 to #6 Largo burs. The apical stops were established at 1 mm (n = 30) and 2 mm (n = 30) from the apex. Final irrigation was performed with 1% NaOCl and 15% EDTA followed by 20 ml of distilled and deionized water. Specimens were subdivided into three subgroups (n = 10 for each stop distance): GI- no radiation (n = 20); GII- 3W potency (n = 20), GIII- 5W potency (n = 20). After preparation, specimens were evaluated by scanning electron microscopy, with ultra-structural changes classified according to a scoring system based on six qualitatively different outcomes. Results: Statistical analysis using the Mann-Whitney test confirmed more intense results for the specimens irradiated at 5 W potency than at 3 W (p < 0.0001). The Kruskal-Wallis test indicated that when using the same potencies (3 or 5 W) at 1 and 2 mm from the apex, there were no statistically significant differences in ultra-structural changes. Conclusions: Our results showed that ultra-structural changes ranged from smear layer removal to dentine fusion. As laser potency was increased from 3 to 5 W, ultra-structural changes included extensive fused lava-like areas sealing the apical foramen.

Original languageEnglish (US)
Pages (from-to)345-349
Number of pages5
JournalPhotomedicine and Laser Surgery
Volume28
Issue number3
DOIs
StatePublished - Jun 1 2010
Externally publishedYes

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Gas Lasers
Dentin
Tooth Apex
Smear Layer
Specimen preparation
Lasers
Deionized water
Dental Pulp Cavity
Ethylenediaminetetraacetic acid
Canals
Laser beam effects
Irrigation
Edetic Acid
Electron Scanning Microscopy
Canidae
Statistical methods
Fusion reactions
Radiation
Scanning electron microscopy
Water

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

Junior, J. G., Marchesan, M., Souza-Gabriel, A. E., Vansan, L. P., Sousa-Neto, M. D., & Silva-Sousa, Y. T. C. (2010). Ultra-structural changes at the apical stop irradiated with CO2 laser. Photomedicine and Laser Surgery, 28(3), 345-349. https://doi.org/10.1089/pho.2008.2473

Ultra-structural changes at the apical stop irradiated with CO2 laser. / Junior, João Gonçalves; Marchesan, Melissa; Souza-Gabriel, Aline Evangelista; Vansan, Luis Pascoal; Sousa-Neto, Manoel D.; Silva-Sousa, Yara T.C.

In: Photomedicine and Laser Surgery, Vol. 28, No. 3, 01.06.2010, p. 345-349.

Research output: Contribution to journalArticle

Junior, JG, Marchesan, M, Souza-Gabriel, AE, Vansan, LP, Sousa-Neto, MD & Silva-Sousa, YTC 2010, 'Ultra-structural changes at the apical stop irradiated with CO2 laser', Photomedicine and Laser Surgery, vol. 28, no. 3, pp. 345-349. https://doi.org/10.1089/pho.2008.2473
Junior JG, Marchesan M, Souza-Gabriel AE, Vansan LP, Sousa-Neto MD, Silva-Sousa YTC. Ultra-structural changes at the apical stop irradiated with CO2 laser. Photomedicine and Laser Surgery. 2010 Jun 1;28(3):345-349. https://doi.org/10.1089/pho.2008.2473
Junior, João Gonçalves ; Marchesan, Melissa ; Souza-Gabriel, Aline Evangelista ; Vansan, Luis Pascoal ; Sousa-Neto, Manoel D. ; Silva-Sousa, Yara T.C. / Ultra-structural changes at the apical stop irradiated with CO2 laser. In: Photomedicine and Laser Surgery. 2010 ; Vol. 28, No. 3. pp. 345-349.
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