Ultrasound prevents renal ischemia-reperfusion injury by stimulating the splenic cholinergic anti-inflammatory pathway

Joseph C. Gigliotti, Liping Huang, Hong Ye, Amandeep Bajwa, Kryt Chattrabhuti, Sangju Lee, Alexander L. Klibanov, Kambiz Kalantari, Diane L. Rosin, Mark D. Okusa

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

AKI affects both quality of life and health care costs and is an independent risk factor for mortality. At present, there are few effective treatment options for AKI. Here, we describe a nonpharmacologic, noninvasive, ultrasound-based method to prevent renal ischemia-reperfusion injury in mice, which is a model for human AKI. We exposed anesthetized mice to an ultrasound protocol 24 hours before renal ischemia. After 24 hours of reperfusion, ultrasound-treated mice exhibited preserved kidney morphology and function compared with sham-treated mice. Ultrasound exposure before renal ischemia reduced the accumulation of CD11b+Ly6Ghigh neutrophils and CD11b+F4/80high myeloid cells in kidney tissue. Furthermore, splenectomy and adoptive transfer studies revealed that the spleen and CD4+ T cellsmediated the protective effects of ultrasound. Last, blockade or genetic deficiency of the a7 nicotinic acetylcholine receptor abrogated the protective effect of ultrasound, suggesting the involvement of the cholinergic anti-inflammatory pathway. Taken together, these results suggest that an ultrasound-based treatment could have therapeutic potential for the prevention of AKI, possibly by stimulating a splenic anti-inflammatory pathway.

Original languageEnglish (US)
Pages (from-to)1451-1460
Number of pages10
JournalJournal of the American Society of Nephrology
Volume24
Issue number9
DOIs
StatePublished - Sep 1 2013

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Reperfusion Injury
Cholinergic Agents
Anti-Inflammatory Agents
Kidney
Ischemia
Adoptive Transfer
Quality of Health Care
Nicotinic Receptors
Splenectomy
Myeloid Cells
Health Care Costs
Reperfusion
Neutrophils
Therapeutics
Spleen
Quality of Life
Mortality

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Ultrasound prevents renal ischemia-reperfusion injury by stimulating the splenic cholinergic anti-inflammatory pathway. / Gigliotti, Joseph C.; Huang, Liping; Ye, Hong; Bajwa, Amandeep; Chattrabhuti, Kryt; Lee, Sangju; Klibanov, Alexander L.; Kalantari, Kambiz; Rosin, Diane L.; Okusa, Mark D.

In: Journal of the American Society of Nephrology, Vol. 24, No. 9, 01.09.2013, p. 1451-1460.

Research output: Contribution to journalArticle

Gigliotti, JC, Huang, L, Ye, H, Bajwa, A, Chattrabhuti, K, Lee, S, Klibanov, AL, Kalantari, K, Rosin, DL & Okusa, MD 2013, 'Ultrasound prevents renal ischemia-reperfusion injury by stimulating the splenic cholinergic anti-inflammatory pathway', Journal of the American Society of Nephrology, vol. 24, no. 9, pp. 1451-1460. https://doi.org/10.1681/ASN.2013010084
Gigliotti, Joseph C. ; Huang, Liping ; Ye, Hong ; Bajwa, Amandeep ; Chattrabhuti, Kryt ; Lee, Sangju ; Klibanov, Alexander L. ; Kalantari, Kambiz ; Rosin, Diane L. ; Okusa, Mark D. / Ultrasound prevents renal ischemia-reperfusion injury by stimulating the splenic cholinergic anti-inflammatory pathway. In: Journal of the American Society of Nephrology. 2013 ; Vol. 24, No. 9. pp. 1451-1460.
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