Ultrastructural double-labeling demonstrates synaptic contacts between dopaminergic terminals and substance P-containing striatal neurons in pigeons

Ellen J. Karle, Keith D. Anderson, Anton Reiner

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Immunohistochemical studies in rats have demonstrated dopaminergic input onto medium spiny neurons of the striatum. Medium spiny neurons, however, are known to consist of two major neuropeptide-specific types, those containing substance P (SP) and those containing enkephalin. Although both of these types have been shown to receive dopaminergic input onto their perikarya and proximal dendrites, the extent to which both types also receive direct dopaminergic input onto distal dendritic shafts or onto dendritic spines is uncertain. In the present study, we used EM immunohistochemical double-label techniques to examine the synaptic organization of dopaminergic input onto SP+ striatal neurons. We examined the striatum of pigeons, in whom SP+ striatal neurons, including their dendritic shafts and spines, can be readily labeled. Antibodies against tyrosine hydroxylase (TH) were used to identify dopaminergic terminals, which were labeled using silver-intensified immunogold. The SP+ neurons were labeled immunohistochemically using diaminobenzidine. We found that dopaminergic terminals make appositions and form symmetric synapses with the perikarya, dendritic shafts and dendritic spines of SP+ neurons. Thus, nigral dopaminergic neurons provide a monosynaptic input onto SP+ striatal neurons in a manner similar to that described for dopaminergic input onto striatal medium spiny neurons in general.

Original languageEnglish (US)
Pages (from-to)303-309
Number of pages7
JournalBrain Research
Volume572
Issue number1-2
DOIs
StatePublished - Feb 14 1992

Fingerprint

Corpus Striatum
Columbidae
Substance P
Neurons
Dendritic Spines
Enkephalins
Dopaminergic Neurons
Tyrosine 3-Monooxygenase
Substantia Nigra
Dendrites
Neuropeptides
Silver
Synapses
Antibodies

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Ultrastructural double-labeling demonstrates synaptic contacts between dopaminergic terminals and substance P-containing striatal neurons in pigeons. / Karle, Ellen J.; Anderson, Keith D.; Reiner, Anton.

In: Brain Research, Vol. 572, No. 1-2, 14.02.1992, p. 303-309.

Research output: Contribution to journalArticle

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