Unamplified RNA Sensor for On-Site Screening of Zika Virus Disease in a Limited Resource Setting

Cheng Cheng, Jayne Wu, Erol Fikrig, Penghua Wang, Jiangang Chen, Shigetoshi Eda, Paul Terry

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Recent outbreaks of Zika virus have been declared a public health emergency of international concern. The diagnosis of Zika infection is based on a person's recent travel history, symptoms, and laboratory test results. However, the diagnosis of Zika infection may be delayed because symptoms are often mild and nondescript, and confirmatory laboratory tests are relatively time-consuming and expensive. Given the lack of an effective vaccine against Zika virus, and a relatively short period of viremia, developing a rapid and sensitive means of detecting the Zika virus in serum is a public health priority. This work presents a novel RNA sensor, based on a sequence-specific probe and AC electrokinetics-enhanced capacitive sensing technology to directly capture and detect Zika virus RNA. This method allows detection and quantification of Zika virus RNA in only 30 seconds, with a low limit of detection (LOD) reaching 158.1 copies/μL. The sensor is also tested for its specificity, showing no false-positive signals from other viruses. In addition, the biosensor is portable, inexpensive, and simple to use, without the need of signal amplification, which makes it ideal for field applications.

Original languageEnglish (US)
Pages (from-to)485-489
Number of pages5
JournalChemElectroChem
Volume4
Issue number3
DOIs
StatePublished - Mar 1 2017

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RNA
Viruses
Screening
Sensors
Public health
Vaccines
Biosensors
Amplification

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electrochemistry

Cite this

Unamplified RNA Sensor for On-Site Screening of Zika Virus Disease in a Limited Resource Setting. / Cheng, Cheng; Wu, Jayne; Fikrig, Erol; Wang, Penghua; Chen, Jiangang; Eda, Shigetoshi; Terry, Paul.

In: ChemElectroChem, Vol. 4, No. 3, 01.03.2017, p. 485-489.

Research output: Contribution to journalArticle

Cheng, Cheng ; Wu, Jayne ; Fikrig, Erol ; Wang, Penghua ; Chen, Jiangang ; Eda, Shigetoshi ; Terry, Paul. / Unamplified RNA Sensor for On-Site Screening of Zika Virus Disease in a Limited Resource Setting. In: ChemElectroChem. 2017 ; Vol. 4, No. 3. pp. 485-489.
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