Unique 5′-end of a Na+-K+-2C1-- cotransporter-like mRNA expressed in rat skeletal muscle

Liusong Fu, Jennifer A. Wong, Edward G. Schneider, Donald Thomason

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Functional evidence presented by others indicates that rat slow-twitch skeletal muscle lacks typical Na+-K+-2Cl- cotransporter activity, as determined by loop diuretic-sensitive potassium transport. This report presents a unique 5′ mRNA sequence of a Na +-K+-2Cl- cotransporter-like molecule expressed in the rat soleus muscle and the deduced N-terminus of the protein. In addition to its unique 5′ mRNA sequence, the coding region of the N-terminus is quite short compared with other known Na+-K+-2Cl -cotransporters. Nonetheless, the mRNA possesses conserved cotransporter-like membrane spanning domains, though one domains corresponding to a reported exon is divergent. Therefore, it appears that skeletal muscle does express a NaC-K+-2Cl- cotransporter-like mRNA that may code for a protein with atypical Na+-K+-2Cl - cotransporter properties.

Original languageEnglish (US)
Pages (from-to)127-132
Number of pages6
JournalMitochondrial DNA
Volume10
Issue number2
DOIs
StatePublished - Dec 1 1999

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Skeletal Muscle
Messenger RNA
Sodium Potassium Chloride Symporter Inhibitors
Exons
Potassium
Proteins
Membranes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Unique 5′-end of a Na+-K+-2C1-- cotransporter-like mRNA expressed in rat skeletal muscle. / Fu, Liusong; Wong, Jennifer A.; Schneider, Edward G.; Thomason, Donald.

In: Mitochondrial DNA, Vol. 10, No. 2, 01.12.1999, p. 127-132.

Research output: Contribution to journalArticle

Fu, Liusong ; Wong, Jennifer A. ; Schneider, Edward G. ; Thomason, Donald. / Unique 5′-end of a Na+-K+-2C1-- cotransporter-like mRNA expressed in rat skeletal muscle. In: Mitochondrial DNA. 1999 ; Vol. 10, No. 2. pp. 127-132.
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