Up-regulation of cytokine mRNA in human monocytes and myeloid cell lines by the differentiation/activation factor p48

Daniel Kestler, S. Agarwal, R. E. Hall

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Polypeptide 48 is a 48,000 MW protein, originally isolated from conditioned media of some human leukaemic cell lines, that induces differentiation and cytolytic activity in HL-60 promyelocytic leukaemia cells and activates human peripheral blood monocytes to secrete interleukin-l (IL-1} and tumour necrosis factor-α (TNF-α). In the present study we examined the effects of p48 on the accumulation of a series of monokine transcripts, including TNF-α, IL-1α, IL-1β and IL-6, in human peripheral blood monocytes and the myeloid/monocyte cell lines HL-60 and U937. Using reverse transcriptase polymerase chain reaction (RT-PCR) and Northern blot analysis, p48 was found to induce accumulation of TNF-α, IL-1α and IL-1β mRNA in peripheral blood monocytes, HL-60 and U937 cells. IL-6 mRNA was found to be increased in p48-stimulated peripheral blood monocytes but not HL-60 or U937. Thus, the secretion of IL-1 and TNF-α by p48-stimulated monocytic cells was associated with up-regulation of cytokine mRNA, suggesting that p48 leads to increased transcription or mRNA stability in these cells. As U937 and HL-60 are likely to represent premonocyte stages of haemopoietic differentiation, it is possible that the effect of p48 on IL-6 mRNA, in contrast to its effect on TNF and IL-1, requires cells to be at a later differentiation step.

Original languageEnglish (US)
Pages (from-to)463-468
Number of pages6
JournalImmunology
Volume86
Issue number3
StatePublished - Jan 1 1995
Externally publishedYes

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Myeloid Cells
Interleukin-1
Monocytes
Cell Differentiation
Up-Regulation
Cytokines
Cell Line
Messenger RNA
Tumor Necrosis Factor-alpha
Interleukin-6
Monokines
U937 Cells
HL-60 Cells
Interleukins
RNA Stability
Conditioned Culture Medium
Reverse Transcriptase Polymerase Chain Reaction
Northern Blotting
Leukemia
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Up-regulation of cytokine mRNA in human monocytes and myeloid cell lines by the differentiation/activation factor p48. / Kestler, Daniel; Agarwal, S.; Hall, R. E.

In: Immunology, Vol. 86, No. 3, 01.01.1995, p. 463-468.

Research output: Contribution to journalArticle

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