Upc2p-associated differential protein expression in Candida albicans

Christopher F. Hoehamer, Edwin D. Cummings, George M. Hilliard, Joachim Morschhäuser, Phillip Rogers

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The gain-of-function mutation G648D in UPC2 causes ERG11 up-regulation and increased fluconazole resistance in Candida albicans. In this study, we performed 2-DE and PMF to identify proteomic alterations in an ERG11-overexpressing fluconazole-resistant C. albicans clinical isolate compared with its fluconazole-susceptible parent strain. We identified 23 differentially expressed proteins, and among them, seven became differentially expressed in a C. albicans wild-type strain after the introduction of a UPC2 allele carrying this mutation. These Upc2p-regulated proteins may contribute to fluconazole resistance in C. albicans.

Original languageEnglish (US)
Pages (from-to)4726-4730
Number of pages5
JournalProteomics
Volume9
Issue number20
DOIs
StatePublished - Oct 20 2009

Fingerprint

Fluconazole
Candida
Candida albicans
Proteins
Mutation
Proteomics
Up-Regulation
Alleles

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Hoehamer, C. F., Cummings, E. D., Hilliard, G. M., Morschhäuser, J., & Rogers, P. (2009). Upc2p-associated differential protein expression in Candida albicans. Proteomics, 9(20), 4726-4730. https://doi.org/10.1002/pmic.200900176

Upc2p-associated differential protein expression in Candida albicans. / Hoehamer, Christopher F.; Cummings, Edwin D.; Hilliard, George M.; Morschhäuser, Joachim; Rogers, Phillip.

In: Proteomics, Vol. 9, No. 20, 20.10.2009, p. 4726-4730.

Research output: Contribution to journalArticle

Hoehamer, CF, Cummings, ED, Hilliard, GM, Morschhäuser, J & Rogers, P 2009, 'Upc2p-associated differential protein expression in Candida albicans', Proteomics, vol. 9, no. 20, pp. 4726-4730. https://doi.org/10.1002/pmic.200900176
Hoehamer CF, Cummings ED, Hilliard GM, Morschhäuser J, Rogers P. Upc2p-associated differential protein expression in Candida albicans. Proteomics. 2009 Oct 20;9(20):4726-4730. https://doi.org/10.1002/pmic.200900176
Hoehamer, Christopher F. ; Cummings, Edwin D. ; Hilliard, George M. ; Morschhäuser, Joachim ; Rogers, Phillip. / Upc2p-associated differential protein expression in Candida albicans. In: Proteomics. 2009 ; Vol. 9, No. 20. pp. 4726-4730.
@article{2f43e569347740008efb2a5e7964bd28,
title = "Upc2p-associated differential protein expression in Candida albicans",
abstract = "The gain-of-function mutation G648D in UPC2 causes ERG11 up-regulation and increased fluconazole resistance in Candida albicans. In this study, we performed 2-DE and PMF to identify proteomic alterations in an ERG11-overexpressing fluconazole-resistant C. albicans clinical isolate compared with its fluconazole-susceptible parent strain. We identified 23 differentially expressed proteins, and among them, seven became differentially expressed in a C. albicans wild-type strain after the introduction of a UPC2 allele carrying this mutation. These Upc2p-regulated proteins may contribute to fluconazole resistance in C. albicans.",
author = "Hoehamer, {Christopher F.} and Cummings, {Edwin D.} and Hilliard, {George M.} and Joachim Morschh{\"a}user and Phillip Rogers",
year = "2009",
month = "10",
day = "20",
doi = "10.1002/pmic.200900176",
language = "English (US)",
volume = "9",
pages = "4726--4730",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "20",

}

TY - JOUR

T1 - Upc2p-associated differential protein expression in Candida albicans

AU - Hoehamer, Christopher F.

AU - Cummings, Edwin D.

AU - Hilliard, George M.

AU - Morschhäuser, Joachim

AU - Rogers, Phillip

PY - 2009/10/20

Y1 - 2009/10/20

N2 - The gain-of-function mutation G648D in UPC2 causes ERG11 up-regulation and increased fluconazole resistance in Candida albicans. In this study, we performed 2-DE and PMF to identify proteomic alterations in an ERG11-overexpressing fluconazole-resistant C. albicans clinical isolate compared with its fluconazole-susceptible parent strain. We identified 23 differentially expressed proteins, and among them, seven became differentially expressed in a C. albicans wild-type strain after the introduction of a UPC2 allele carrying this mutation. These Upc2p-regulated proteins may contribute to fluconazole resistance in C. albicans.

AB - The gain-of-function mutation G648D in UPC2 causes ERG11 up-regulation and increased fluconazole resistance in Candida albicans. In this study, we performed 2-DE and PMF to identify proteomic alterations in an ERG11-overexpressing fluconazole-resistant C. albicans clinical isolate compared with its fluconazole-susceptible parent strain. We identified 23 differentially expressed proteins, and among them, seven became differentially expressed in a C. albicans wild-type strain after the introduction of a UPC2 allele carrying this mutation. These Upc2p-regulated proteins may contribute to fluconazole resistance in C. albicans.

UR - http://www.scopus.com/inward/record.url?scp=70350239846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350239846&partnerID=8YFLogxK

U2 - 10.1002/pmic.200900176

DO - 10.1002/pmic.200900176

M3 - Article

C2 - 19750515

AN - SCOPUS:70350239846

VL - 9

SP - 4726

EP - 4730

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 20

ER -