Upregulation of PD-L1 via HMGB1-activated IRF3 and NF-kB contributes to UV radiation-induced immune suppression

Wei Wang, Nicole M. Chapman, Bo Zhang, Mingqi Li, Meiyun Fan, Ronald Laribee, M. Raza Zaidi, Lawrence Pfeffer, Hongbo Chi, Zhaohui Wu

Research output: Contribution to journalArticle

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Abstract

Solar ultraviolet radiation (UVR) suppresses skin immunity, which facilitates initiation of skin lesions and establishment of tumors by promoting immune evasion. It is unclear whether immune checkpoints are involved in the modulation of skin immunity by UVR. Here, we report that UVR exposure significantly increased expression of immune checkpoint molecule PD-L1 in melanoma cells. The damage-associated molecular patterns molecule HMGB1 was secreted by melanocytes and keratinocytes upon UVR, which subsequently activated the receptor for advanced glycation endproducts (RAGE) receptor to promote NF-kB– and IRF3-dependent transcription of PD-L1 in melanocytes. UVR exposure significantly reduced the susceptibility of melanoma cells to CD8þ T-cell–dependent cytotoxicity, which was mitigated by inhibiting the HMGB1/ TBK1/IRF3/NF-kB cascade or by blocking the PD-1/PD-L1 checkpoint. Taken together, our findings demonstrate that UVR-induced upregulation of PD-L1 contributes to immune suppression in the skin microenvironment, which may promote immune evasion of oncogenic cells and drive melanoma initiation and progression. Significance: These findings identify PD-L1 as a critical component of UV-induced immune suppression in the skin, which facilitates immunoevasion of oncogenic melanocytes and development of melanoma.

Original languageEnglish (US)
Pages (from-to)2909-2922
Number of pages14
JournalCancer Research
Volume79
Issue number11
DOIs
StatePublished - Jun 1 2019

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HMGB1 Protein
NF-kappa B
Up-Regulation
Radiation
Melanocytes
Melanoma
Skin
Immunity
Tumor Escape
Immune Evasion
Keratinocytes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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Upregulation of PD-L1 via HMGB1-activated IRF3 and NF-kB contributes to UV radiation-induced immune suppression. / Wang, Wei; Chapman, Nicole M.; Zhang, Bo; Li, Mingqi; Fan, Meiyun; Laribee, Ronald; Raza Zaidi, M.; Pfeffer, Lawrence; Chi, Hongbo; Wu, Zhaohui.

In: Cancer Research, Vol. 79, No. 11, 01.06.2019, p. 2909-2922.

Research output: Contribution to journalArticle

Wang, Wei ; Chapman, Nicole M. ; Zhang, Bo ; Li, Mingqi ; Fan, Meiyun ; Laribee, Ronald ; Raza Zaidi, M. ; Pfeffer, Lawrence ; Chi, Hongbo ; Wu, Zhaohui. / Upregulation of PD-L1 via HMGB1-activated IRF3 and NF-kB contributes to UV radiation-induced immune suppression. In: Cancer Research. 2019 ; Vol. 79, No. 11. pp. 2909-2922.
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abstract = "Solar ultraviolet radiation (UVR) suppresses skin immunity, which facilitates initiation of skin lesions and establishment of tumors by promoting immune evasion. It is unclear whether immune checkpoints are involved in the modulation of skin immunity by UVR. Here, we report that UVR exposure significantly increased expression of immune checkpoint molecule PD-L1 in melanoma cells. The damage-associated molecular patterns molecule HMGB1 was secreted by melanocytes and keratinocytes upon UVR, which subsequently activated the receptor for advanced glycation endproducts (RAGE) receptor to promote NF-kB– and IRF3-dependent transcription of PD-L1 in melanocytes. UVR exposure significantly reduced the susceptibility of melanoma cells to CD8{\th} T-cell–dependent cytotoxicity, which was mitigated by inhibiting the HMGB1/ TBK1/IRF3/NF-kB cascade or by blocking the PD-1/PD-L1 checkpoint. Taken together, our findings demonstrate that UVR-induced upregulation of PD-L1 contributes to immune suppression in the skin microenvironment, which may promote immune evasion of oncogenic cells and drive melanoma initiation and progression. Significance: These findings identify PD-L1 as a critical component of UV-induced immune suppression in the skin, which facilitates immunoevasion of oncogenic melanocytes and development of melanoma.",
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