Urea carboxylase and allophanate hydrolase

Two components of a multienzyme complex in Saccharomyces cerevisiae

P. A. Whitney, Terrance Cooper

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Saccharomyces cerevisiae can utilize urea as sole nitrogen source by virtue of its ability to degrade the compound to NH3 and CO2. Whitney and Cooper have shown that the conversion is accomplished in two enzymatic steps: (1) the carboxylation of urea yielding allophanate and (2) the hydrolysis of allophanate yielding NH3 and CO2. To determine whether these proteins compose a multienzyme complex, the response of urea carboxylase and allophanate hydrolase to several analytical separation and immunochemical precipitation techniques was monitored. The data obtained support the contention that urea carboxylase and allophanate hydrolase are components of a multienzyme complex.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume49
Issue number1
DOIs
StatePublished - Oct 6 1972

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Allophanate Hydrolase
Multienzyme Complexes
Yeast
Saccharomyces cerevisiae
Urea
Carboxylation
Hydrolysis
Nitrogen
Proteins
urea carboxylase (hydrolyzing)
allophanic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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AB - Saccharomyces cerevisiae can utilize urea as sole nitrogen source by virtue of its ability to degrade the compound to NH3 and CO2. Whitney and Cooper have shown that the conversion is accomplished in two enzymatic steps: (1) the carboxylation of urea yielding allophanate and (2) the hydrolysis of allophanate yielding NH3 and CO2. To determine whether these proteins compose a multienzyme complex, the response of urea carboxylase and allophanate hydrolase to several analytical separation and immunochemical precipitation techniques was monitored. The data obtained support the contention that urea carboxylase and allophanate hydrolase are components of a multienzyme complex.

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