Urea transport defective strains of Saccharomyces cerevisiae

R. Sumrada, M. Gorski, Terrance Cooper

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Experiments characterizing the urea active transport system in S. cerevisiae indicate that formamide and acetamide are strong competitive inhibitors of urea accumulation, uptake is maximal at pH 3.3 and is 80% inhibited at pH 6.0 and adenosine 5' triphosphate generated by glycolysis in conjunction with formation of an ion gradient is likely the driving force behind urea transport. Mutant strains were isolated that are unable to accumulate urea at external concentrations of 0.25 mM. These strains also exhibit a depressed growth rate on 10mM urea, indicating existence of a relationship between the active transport and facilitated diffusion modes of urea uptake.

Original languageEnglish (US)
Pages (from-to)1048-1056
Number of pages9
JournalJournal of bacteriology
Volume125
Issue number3
StatePublished - Dec 1 1976

Fingerprint

Saccharomyces cerevisiae
Urea
Active Biological Transport
Facilitated Diffusion
Glycolysis
Adenosine Triphosphate
Ions
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Urea transport defective strains of Saccharomyces cerevisiae. / Sumrada, R.; Gorski, M.; Cooper, Terrance.

In: Journal of bacteriology, Vol. 125, No. 3, 01.12.1976, p. 1048-1056.

Research output: Contribution to journalArticle

Sumrada, R, Gorski, M & Cooper, T 1976, 'Urea transport defective strains of Saccharomyces cerevisiae', Journal of bacteriology, vol. 125, no. 3, pp. 1048-1056.
Sumrada, R. ; Gorski, M. ; Cooper, Terrance. / Urea transport defective strains of Saccharomyces cerevisiae. In: Journal of bacteriology. 1976 ; Vol. 125, No. 3. pp. 1048-1056.
@article{26dc1d6524d047c7bf2ab1fd8c1c49b6,
title = "Urea transport defective strains of Saccharomyces cerevisiae",
abstract = "Experiments characterizing the urea active transport system in S. cerevisiae indicate that formamide and acetamide are strong competitive inhibitors of urea accumulation, uptake is maximal at pH 3.3 and is 80{\%} inhibited at pH 6.0 and adenosine 5' triphosphate generated by glycolysis in conjunction with formation of an ion gradient is likely the driving force behind urea transport. Mutant strains were isolated that are unable to accumulate urea at external concentrations of 0.25 mM. These strains also exhibit a depressed growth rate on 10mM urea, indicating existence of a relationship between the active transport and facilitated diffusion modes of urea uptake.",
author = "R. Sumrada and M. Gorski and Terrance Cooper",
year = "1976",
month = "12",
day = "1",
language = "English (US)",
volume = "125",
pages = "1048--1056",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Urea transport defective strains of Saccharomyces cerevisiae

AU - Sumrada, R.

AU - Gorski, M.

AU - Cooper, Terrance

PY - 1976/12/1

Y1 - 1976/12/1

N2 - Experiments characterizing the urea active transport system in S. cerevisiae indicate that formamide and acetamide are strong competitive inhibitors of urea accumulation, uptake is maximal at pH 3.3 and is 80% inhibited at pH 6.0 and adenosine 5' triphosphate generated by glycolysis in conjunction with formation of an ion gradient is likely the driving force behind urea transport. Mutant strains were isolated that are unable to accumulate urea at external concentrations of 0.25 mM. These strains also exhibit a depressed growth rate on 10mM urea, indicating existence of a relationship between the active transport and facilitated diffusion modes of urea uptake.

AB - Experiments characterizing the urea active transport system in S. cerevisiae indicate that formamide and acetamide are strong competitive inhibitors of urea accumulation, uptake is maximal at pH 3.3 and is 80% inhibited at pH 6.0 and adenosine 5' triphosphate generated by glycolysis in conjunction with formation of an ion gradient is likely the driving force behind urea transport. Mutant strains were isolated that are unable to accumulate urea at external concentrations of 0.25 mM. These strains also exhibit a depressed growth rate on 10mM urea, indicating existence of a relationship between the active transport and facilitated diffusion modes of urea uptake.

UR - http://www.scopus.com/inward/record.url?scp=0017235579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017235579&partnerID=8YFLogxK

M3 - Article

VL - 125

SP - 1048

EP - 1056

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 3

ER -