Ureaplasma urealyticum binds mannose-binding lectin

Barbara Dubray-Benstein, Donald D. Ourth, Dennis T. Crouse, D. Radford Shanklin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Mannose-binding C-type lectin (MBL) is an important component of innate immunity in mammals. Mannose-binding lectin (MBL), an acute phase protein, acts as an opsonin for phagocytosis and also activates the mannan-binding lectin complement pathway. It may play a particularly significant role during infancy before adequate specific protection can be provided by the adaptive immune system. Ureaplasma urealyticum has been linked to several diseases including pneumonia and chronic lung disease (CLD) in premature infants. We therefore investigated the ability of U. urealyticum to bind MBL. A guinea pig IgG anti-rabbit-MBL antiserum was produced. An immunoblot (dot-blot) assay done on nitrocellulose membrane determined that the anti-MBL antibody had specificity against both rabbit and human MBL. Pure cultures of U. urealyticum, serotype 3, were used to make slide preparations. The slides containing the organisms were then incubated with nonimmune rabbit serum containing MBL. Ureaplasma was shown to bind rabbit MBL with an immunocytochemical assay using the guinea pig IgG anti-rabbit MBL antiserum. Horseradish peroxidase (HRP)-labeled anti-guinea pig IgG was used to localize the reaction. The anti-MBL antiserum was also used in an immunocytochemical assay to localize U. urealyticum in histological sections of lungs from mice specifically infected with this organism. The same method also indicated binding of MBL by ureaplasma in human lung tissue obtained at autopsy from culture positive infants. Our results demonstrate that ureaplasma has the capacity to bind MBL. The absence of MBL may play a role in the predisposition of diseases related to this organism.

Original languageEnglish (US)
Pages (from-to)138-144
Number of pages7
JournalExperimental and Molecular Pathology
Volume77
Issue number2
DOIs
StatePublished - Oct 1 2004

Fingerprint

Ureaplasma urealyticum
Mannose-Binding Lectin
Ureaplasma
Rabbits
Immune Sera
Assays
Guinea Pigs
Mannose-Binding Lectin Complement Pathway
Opsonin Proteins
C-Type Lectins
Lung
Pulmonary diseases
Mammals
Collodion
Antibody Specificity
Acute-Phase Proteins
Immune system
Horseradish Peroxidase
Mannose
Phagocytosis

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

Ureaplasma urealyticum binds mannose-binding lectin. / Dubray-Benstein, Barbara; Ourth, Donald D.; Crouse, Dennis T.; Radford Shanklin, D.

In: Experimental and Molecular Pathology, Vol. 77, No. 2, 01.10.2004, p. 138-144.

Research output: Contribution to journalArticle

Dubray-Benstein, Barbara ; Ourth, Donald D. ; Crouse, Dennis T. ; Radford Shanklin, D. / Ureaplasma urealyticum binds mannose-binding lectin. In: Experimental and Molecular Pathology. 2004 ; Vol. 77, No. 2. pp. 138-144.
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