Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease

Krishna C. Keri, Kevin R. Regner, Aaron T. Dall, Frank Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: PKD is a genetic disease that is characterized by abnormally proliferative epithelial cells in the kidney and liver. Urinary exosomes have been previously examined as a source of unique proteins that may be used to diagnose and monitor the progression of PKD. Previous studies by our group have shown that AGS3, which is a receptor-independent regulator G-proteins, was markedly upregulated in RTECs during kidney injury including PKD. In this study, our goal was to determine whether AGS3 could be measured in exosomes using animals and humans with PKD. Results: In our study, urinary exosomes were isolated from PCK rats and the control Sprague-Dawley (SD) rats. AGS3 expression was significantly increased (P < 0.05) in PKD versus SD rats at 16 weeks of age. This increase was detectable in a time-dependent manner from 8 weeks of age and peaked at ~ 16-20 weeks (length of study). Similarly, in exosomes from human urine samples with PKD, AGS3 expression was significantly increased (P < 0.05) compared to healthy human controls where AGS3 was largely undetectable. In conclusion, the detection of AGS3 in urinary exosomes may be a novel biomarker for PKD, and provide new insight into the biology of tubular epithelial cell function during cystic disease progression.

Original languageEnglish (US)
Article number359
JournalBMC Research Notes
Volume11
Issue number1
DOIs
StatePublished - Jun 7 2018

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Exosomes
Polycystic Kidney Diseases
GTP-Binding Proteins
Rats
Biomarkers
Sprague Dawley Rats
Liver
Epithelial Cells
Animals
Kidney
Inborn Genetic Diseases
Disease Progression
Urine
Proteins
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease. / Keri, Krishna C.; Regner, Kevin R.; Dall, Aaron T.; Park, Frank.

In: BMC Research Notes, Vol. 11, No. 1, 359, 07.06.2018.

Research output: Contribution to journalArticle

Keri, Krishna C. ; Regner, Kevin R. ; Dall, Aaron T. ; Park, Frank. / Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease. In: BMC Research Notes. 2018 ; Vol. 11, No. 1.
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