Urinary Isothiocyanate Excretion, Brassica Consumption, and Gene Polymorphisms among Women Living in Shanghai, China

Jay Fowke, Xiao Ou Shu, Qi Dai, Ayumi Shintani, C. Clifford Conaway, Fung Lung Chung, Qiuyin Cai, Yu Tang Gao, Wei Zheng

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Alternative measures of Brassica vegetable consumption (e.g., cabbage) may clarify the association between Brassica and cancer risk. Brassica isothiocyanates (ITCs) are excreted in urine and may provide a sensitive and food-specific dietary biomarker. However, the persistence of ITCs in the body may be brief and dependent on the activity of several Phase II enzymes, raising questions about the relationship between a single ITC measure and habitual dietary patterns. This study investigates the association between urinary ITC excretion and habitual Brassica consumption, estimated by a food frequency questionnaire, among healthy Chinese women enrolled in the Shanghai Breast Cancer Study. Participants (n = 347) completed a validated food frequency questionnaire querying habitual dietary intake during the prior 5 years and provided a fasting first-morning urine specimen. Genetic deletion of glutathione S-transferases (GSTM1/GSTT1), and single nucleotide substitutions in GSTP1 (A313G) and NAD(P)H:quinone oxidoreductase 1 (NQ01: C609T), were identified from blood DNA. Urinary ITC excretion levels were marginally higher with the GSTT1-null or GSTP1-G/G genotypes (P = 0.07, P = 0.05, respectively). Mean habitual Brassica intake was 98.3 g/day, primarily as bok choy, and Brassica intake significantly increased across quartile categories of ITC levels. The association between habitual Brassica intake and urinary ITC levels was stronger among women with GSTT1-null or GSTP1-A/A genotypes, or NQO1 T-allele, and the interaction was statistically significant across GSTP1 genotype. In conclusion, a single urinary ITC measure, in conjunction with markers of Phase II enzyme activity, provides a complementary measure of habitual Brassica intake among Shanghai women.

Original languageEnglish (US)
Pages (from-to)1536-1539
Number of pages4
JournalCancer Epidemiology Biomarkers and Prevention
Volume12
Issue number12
StatePublished - Dec 1 2003
Externally publishedYes

Fingerprint

Isothiocyanates
Brassica
China
Genes
Genotype
Food
Urine
isothiocyanic acid
Enzymes
Vegetables
NAD
Fasting
Oxidoreductases
Nucleotides
Biomarkers
Alleles
Breast Neoplasms
DNA

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology

Cite this

Urinary Isothiocyanate Excretion, Brassica Consumption, and Gene Polymorphisms among Women Living in Shanghai, China. / Fowke, Jay; Shu, Xiao Ou; Dai, Qi; Shintani, Ayumi; Conaway, C. Clifford; Chung, Fung Lung; Cai, Qiuyin; Gao, Yu Tang; Zheng, Wei.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 12, No. 12, 01.12.2003, p. 1536-1539.

Research output: Contribution to journalArticle

Fowke, J, Shu, XO, Dai, Q, Shintani, A, Conaway, CC, Chung, FL, Cai, Q, Gao, YT & Zheng, W 2003, 'Urinary Isothiocyanate Excretion, Brassica Consumption, and Gene Polymorphisms among Women Living in Shanghai, China', Cancer Epidemiology Biomarkers and Prevention, vol. 12, no. 12, pp. 1536-1539.
Fowke, Jay ; Shu, Xiao Ou ; Dai, Qi ; Shintani, Ayumi ; Conaway, C. Clifford ; Chung, Fung Lung ; Cai, Qiuyin ; Gao, Yu Tang ; Zheng, Wei. / Urinary Isothiocyanate Excretion, Brassica Consumption, and Gene Polymorphisms among Women Living in Shanghai, China. In: Cancer Epidemiology Biomarkers and Prevention. 2003 ; Vol. 12, No. 12. pp. 1536-1539.
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abstract = "Alternative measures of Brassica vegetable consumption (e.g., cabbage) may clarify the association between Brassica and cancer risk. Brassica isothiocyanates (ITCs) are excreted in urine and may provide a sensitive and food-specific dietary biomarker. However, the persistence of ITCs in the body may be brief and dependent on the activity of several Phase II enzymes, raising questions about the relationship between a single ITC measure and habitual dietary patterns. This study investigates the association between urinary ITC excretion and habitual Brassica consumption, estimated by a food frequency questionnaire, among healthy Chinese women enrolled in the Shanghai Breast Cancer Study. Participants (n = 347) completed a validated food frequency questionnaire querying habitual dietary intake during the prior 5 years and provided a fasting first-morning urine specimen. Genetic deletion of glutathione S-transferases (GSTM1/GSTT1), and single nucleotide substitutions in GSTP1 (A313G) and NAD(P)H:quinone oxidoreductase 1 (NQ01: C609T), were identified from blood DNA. Urinary ITC excretion levels were marginally higher with the GSTT1-null or GSTP1-G/G genotypes (P = 0.07, P = 0.05, respectively). Mean habitual Brassica intake was 98.3 g/day, primarily as bok choy, and Brassica intake significantly increased across quartile categories of ITC levels. The association between habitual Brassica intake and urinary ITC levels was stronger among women with GSTT1-null or GSTP1-A/A genotypes, or NQO1 T-allele, and the interaction was statistically significant across GSTP1 genotype. In conclusion, a single urinary ITC measure, in conjunction with markers of Phase II enzyme activity, provides a complementary measure of habitual Brassica intake among Shanghai women.",
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AU - Conaway, C. Clifford

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