Urinary microbiota associated with preterm birth

Results from the conditions affecting neurocognitive development and learning in early childhood (CANDLE) study

Nicholas J. Ollberding, Eszter Völgyi, Maurizio Macaluso, Ranjit Kumar, Casey Morrow, Frances Tylavsky, Chandrika J. Piyathilake

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Preterm birth (PTB) is the leading cause of infant morbidity and mortality. Genitourinary infection is implicated in the initiation of spontaneous PTB; however, examination of the urinary microbiota in relation to preterm delivery using next-generation sequencing technologies is lacking. In a case-control study nested within the Conditions Affecting Neurocognitive Development and Learning in Early Childhood (CANDLE) study, we examined associations between the urinary microbiota and PTB. A total of 49 cases (delivery < 37 weeks gestation) and 48 controls (delivery ≥ 37 weeks gestation) balanced on health insurance type were included in the present analysis. Illumina sequencing of the 16S rRNA gene V4 region was performed on urine samples collected during the second trimester. We observed no difference in taxa richness, evenness, or community composition between cases and controls or for gestational age modeled as a continuous variable. Operational taxonomic units (OTUs) classified to Prevotella, Sutterella, L. iners, Blautia, Kocuria, Lachnospiraceae, and S.marcescens were enriched among cases (FDR corrected p≤ 0.05). A urinary microbiota clustering partition dominated by S. marcescens was also associated with PTB (OR = 3.97, 95% CI: 1.19'13.24). These data suggest a limited role for the urinary microbiota in PTB when measured during the second trimester by 16S rRNA gene sequencing. The enrichment among cases in several organisms previously reported to be associated with genitourinary pathology requires confirmation in future studies to rule out the potential for false positive findings.

Original languageEnglish (US)
Article numbere0162302
JournalPloS one
Volume11
Issue number9
DOIs
StatePublished - Sep 1 2016

Fingerprint

premature birth
Microbiota
Premature Birth
childhood
learning
Learning
Genes
Health insurance
Second Pregnancy Trimester
rRNA Genes
Sutterella
Pathology
Kocuria
ribosomal RNA
pregnancy
Prevotella
health insurance
Pregnancy
gestational age
Infant Mortality

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Urinary microbiota associated with preterm birth : Results from the conditions affecting neurocognitive development and learning in early childhood (CANDLE) study. / Ollberding, Nicholas J.; Völgyi, Eszter; Macaluso, Maurizio; Kumar, Ranjit; Morrow, Casey; Tylavsky, Frances; Piyathilake, Chandrika J.

In: PloS one, Vol. 11, No. 9, e0162302, 01.09.2016.

Research output: Contribution to journalArticle

Ollberding, Nicholas J. ; Völgyi, Eszter ; Macaluso, Maurizio ; Kumar, Ranjit ; Morrow, Casey ; Tylavsky, Frances ; Piyathilake, Chandrika J. / Urinary microbiota associated with preterm birth : Results from the conditions affecting neurocognitive development and learning in early childhood (CANDLE) study. In: PloS one. 2016 ; Vol. 11, No. 9.
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