Use of Isotope Tracers to Assess Lipid Absorption in Conscious Lymph Fistula Mice

Chih Wei Ko, Jie Qu, Min Liu, Dennis Black, Patrick Tso

Research output: Contribution to journalArticle

Abstract

This protocol provides a comprehensive reference for the evolution of the lymph fistula model, the mechanism of lipid absorption, the detailed procedure for studying lipid absorption using the lymph fistula model, the interpretation of the results, and consideration of the experimental design. The lymph fistula model is an approach to assess the concentration and rate of a range of molecules transported by the lymph by cannulating lymph duct in animals. In this protocol, mice first undergo surgery with the implantation of cannulae in the duodenum and mesenteric lymph duct and are allowed to recover overnight in Bollman restraining cages housed in a temperature-regulated environment. To study in vivo lipid absorption, a lipid emulsion is prepared with labeled tracers, including [3 H]-triolein and [14 C]-cholesterol. On the day of the experiment, mice are continuously infused with lipid emulsion via the duodenum for 6 hr, and lymph is usually collected hourly. At the end of the study, gastrointestinal segments and their luminal contents are collected separately for determination of the digestion, uptake, and transport of exogenous lipids.

Original languageEnglish (US)
Pages (from-to)e60
JournalCurrent protocols in mouse biology
Volume9
Issue number1
DOIs
StatePublished - Mar 1 2019

Fingerprint

Lymph
Isotopes
Fistula
Lipids
Emulsions
Duodenum
Triolein
Digestion
Research Design
Cholesterol
Temperature

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Use of Isotope Tracers to Assess Lipid Absorption in Conscious Lymph Fistula Mice. / Ko, Chih Wei; Qu, Jie; Liu, Min; Black, Dennis; Tso, Patrick.

In: Current protocols in mouse biology, Vol. 9, No. 1, 01.03.2019, p. e60.

Research output: Contribution to journalArticle

Ko, Chih Wei ; Qu, Jie ; Liu, Min ; Black, Dennis ; Tso, Patrick. / Use of Isotope Tracers to Assess Lipid Absorption in Conscious Lymph Fistula Mice. In: Current protocols in mouse biology. 2019 ; Vol. 9, No. 1. pp. e60.
@article{f9243cb321524aadbd227ebbee1167d1,
title = "Use of Isotope Tracers to Assess Lipid Absorption in Conscious Lymph Fistula Mice",
abstract = "This protocol provides a comprehensive reference for the evolution of the lymph fistula model, the mechanism of lipid absorption, the detailed procedure for studying lipid absorption using the lymph fistula model, the interpretation of the results, and consideration of the experimental design. The lymph fistula model is an approach to assess the concentration and rate of a range of molecules transported by the lymph by cannulating lymph duct in animals. In this protocol, mice first undergo surgery with the implantation of cannulae in the duodenum and mesenteric lymph duct and are allowed to recover overnight in Bollman restraining cages housed in a temperature-regulated environment. To study in vivo lipid absorption, a lipid emulsion is prepared with labeled tracers, including [3 H]-triolein and [14 C]-cholesterol. On the day of the experiment, mice are continuously infused with lipid emulsion via the duodenum for 6 hr, and lymph is usually collected hourly. At the end of the study, gastrointestinal segments and their luminal contents are collected separately for determination of the digestion, uptake, and transport of exogenous lipids.",
author = "Ko, {Chih Wei} and Jie Qu and Min Liu and Dennis Black and Patrick Tso",
year = "2019",
month = "3",
day = "1",
doi = "10.1002/cpmo.60",
language = "English (US)",
volume = "9",
pages = "e60",
journal = "Current protocols in mouse biology",
issn = "2161-2617",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Use of Isotope Tracers to Assess Lipid Absorption in Conscious Lymph Fistula Mice

AU - Ko, Chih Wei

AU - Qu, Jie

AU - Liu, Min

AU - Black, Dennis

AU - Tso, Patrick

PY - 2019/3/1

Y1 - 2019/3/1

N2 - This protocol provides a comprehensive reference for the evolution of the lymph fistula model, the mechanism of lipid absorption, the detailed procedure for studying lipid absorption using the lymph fistula model, the interpretation of the results, and consideration of the experimental design. The lymph fistula model is an approach to assess the concentration and rate of a range of molecules transported by the lymph by cannulating lymph duct in animals. In this protocol, mice first undergo surgery with the implantation of cannulae in the duodenum and mesenteric lymph duct and are allowed to recover overnight in Bollman restraining cages housed in a temperature-regulated environment. To study in vivo lipid absorption, a lipid emulsion is prepared with labeled tracers, including [3 H]-triolein and [14 C]-cholesterol. On the day of the experiment, mice are continuously infused with lipid emulsion via the duodenum for 6 hr, and lymph is usually collected hourly. At the end of the study, gastrointestinal segments and their luminal contents are collected separately for determination of the digestion, uptake, and transport of exogenous lipids.

AB - This protocol provides a comprehensive reference for the evolution of the lymph fistula model, the mechanism of lipid absorption, the detailed procedure for studying lipid absorption using the lymph fistula model, the interpretation of the results, and consideration of the experimental design. The lymph fistula model is an approach to assess the concentration and rate of a range of molecules transported by the lymph by cannulating lymph duct in animals. In this protocol, mice first undergo surgery with the implantation of cannulae in the duodenum and mesenteric lymph duct and are allowed to recover overnight in Bollman restraining cages housed in a temperature-regulated environment. To study in vivo lipid absorption, a lipid emulsion is prepared with labeled tracers, including [3 H]-triolein and [14 C]-cholesterol. On the day of the experiment, mice are continuously infused with lipid emulsion via the duodenum for 6 hr, and lymph is usually collected hourly. At the end of the study, gastrointestinal segments and their luminal contents are collected separately for determination of the digestion, uptake, and transport of exogenous lipids.

UR - http://www.scopus.com/inward/record.url?scp=85062608782&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062608782&partnerID=8YFLogxK

U2 - 10.1002/cpmo.60

DO - 10.1002/cpmo.60

M3 - Article

VL - 9

SP - e60

JO - Current protocols in mouse biology

JF - Current protocols in mouse biology

SN - 2161-2617

IS - 1

ER -