Use of replication restricted recombinant vesicular stomatitis virus vectors for detection of antigen-specific T cells

Nelson B. Moseley, Oskar Laur, Chris C. Ibegbu, Gilbert D. Loria, Gini Ikwuenzunma, Himangi R. Jayakar, Michael Whitt, John D. Altman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Detection of antigen-specific T cells at the single-cell level by ELISpot or flow cytometry techniques employing intracellular cytokine staining (ICS) is now an indispensable tool in many areas of immunology. When precisely mapped, optimal MHC-binding peptide epitopes are unknown, these assays use antigen in a variety of forms, including recombinant proteins, overlapping peptide sets representing one or more target protein sequences, microbial lysates, lysates of microbially-infected cells, or gene delivery vectors such as DNA expression plasmids or recombinant vaccinia or adenoviruses expressing a target protein of interest. Here we introduce replication-restricted, recombinant vesicular stomatitis virus (VSV) vectors as a safe, easy to produce, simple to use, and highly effective vector for genetic antigen delivery for the detection of human antigen-specific helper and cytotoxic T cells. To demonstrate the broad applicability of this approach, we have used these vectors to detect human T cell responses to the immunodominant pp65 antigen of human cytomegalovirus, individual segments of the yellow fever virus polyprotein, and to various influenza proteins.

Original languageEnglish (US)
Pages (from-to)118-128
Number of pages11
JournalJournal of Immunological Methods
Volume375
Issue number1-2
DOIs
StatePublished - Jan 31 2012

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Vesicular Stomatitis
Viruses
T-Lymphocytes
Antigens
Yellow fever virus
Polyproteins
Vaccinia
Immunodominant Epitopes
Genetic Vectors
Proteins
Helper-Inducer T-Lymphocytes
Allergy and Immunology
Cytomegalovirus
Recombinant Proteins
Adenoviridae
Human Influenza
Epitopes
Flow Cytometry
Plasmids
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Moseley, N. B., Laur, O., Ibegbu, C. C., Loria, G. D., Ikwuenzunma, G., Jayakar, H. R., ... Altman, J. D. (2012). Use of replication restricted recombinant vesicular stomatitis virus vectors for detection of antigen-specific T cells. Journal of Immunological Methods, 375(1-2), 118-128. https://doi.org/10.1016/j.jim.2011.09.016

Use of replication restricted recombinant vesicular stomatitis virus vectors for detection of antigen-specific T cells. / Moseley, Nelson B.; Laur, Oskar; Ibegbu, Chris C.; Loria, Gilbert D.; Ikwuenzunma, Gini; Jayakar, Himangi R.; Whitt, Michael; Altman, John D.

In: Journal of Immunological Methods, Vol. 375, No. 1-2, 31.01.2012, p. 118-128.

Research output: Contribution to journalArticle

Moseley, Nelson B. ; Laur, Oskar ; Ibegbu, Chris C. ; Loria, Gilbert D. ; Ikwuenzunma, Gini ; Jayakar, Himangi R. ; Whitt, Michael ; Altman, John D. / Use of replication restricted recombinant vesicular stomatitis virus vectors for detection of antigen-specific T cells. In: Journal of Immunological Methods. 2012 ; Vol. 375, No. 1-2. pp. 118-128.
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