Uteroglobin gene expression in the rabbit uterus throughout gestation and in the fetal lung. Relationship between uteroglobin and eicosanoid levels in the developing fetal lung

A. Peri, N. H. Dubin, Ramasubbareddy Dhanireddy, A. B. Mukherjee

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Uteroglobin (UG) gene encodes a cytokine-like, multifunctional, antiinflammatory protein, with potent phospholipase A2-inhibitory activity. It has been suggested that during implantation this protein protects the embryos from maternal immunological assault, facilitates the maintenance of quiescence in the uterus throughout pregnancy, prevents the onset of premature labor, and helps maintain an inflammation-free respiratory organ. This latter function of UG is suggested to be accomplished by preventing hydrolysis of surfactant phospholipids by a lung-specific phospholipase A2. Using reverse transcription polymerase chain reaction, in situ hybridization, immunofluorescence, and radioimmunoassay, we studied UG gene expression in the rabbit uterus throughout gestation and in the fetal lung. Here, we report that: (a) contrary to previous reports, UG gene expression in the rabbit uterus occurs throughout gestation with a precipitous decline just before parturition; (b) this gene expression is dramatically increased in the fetal lung with increasing gestational age; and (c) while there is an inverse relationship between the levels of UG, PGE2, and PGF(2α), a positive correlation was found in that of UG and leukotriene C4 in the fetal lung. Our results raise the possibility that dysregulation of UG gene expression, at least in part, may contribute to the onset of premature labor and the development of inflammatory lung disease in premature neonates.

Original languageEnglish (US)
Pages (from-to)343-353
Number of pages11
JournalJournal of Clinical Investigation
Volume96
Issue number1
DOIs
StatePublished - Jan 1 1995

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Uteroglobin
Eicosanoids
Uterus
Rabbits
Gene Expression
Pregnancy
Lung
Premature Obstetric Labor
Phospholipases A2
Leukotriene C4
Prostaglandins F
Dinoprostone
Surface-Active Agents
Lung Diseases
Gestational Age
Reverse Transcription
Radioimmunoassay
In Situ Hybridization
Fluorescent Antibody Technique
Phospholipids

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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AU - Mukherjee, A. B.

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