Utility of α-methylacyl coenzyme A racemase (P504S antibody) as a diagnostic immunohistochemical marker for cancer

Aziza Nassar, Mahul Amin, Deborah G. Sexton, Cynthia Cohen

Research output: Contribution to journalArticle

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Abstract

α-Methylacyl-coenzyme A racemase (AMACR; P504S) is a mitochondrial and peroxisomal enzyme involved in the metabolism of branched-chain fatty acid and bile acid intermediates. Recently, AMACR has been demonstrated to be overexpressed in localized and metastatic prostate cancer and in high-grade prostatic intraepithelial neoplasia but not in normal prostatic glands, suggesting that it may be an important tumor marker. This study examines AMACR expression in a variety of human cancers to assess its viability as a tumor marker in the clinical setting. Two hundred sixty-three cancers from different sites were examined in three multitumor tissue micro arrays, which included two or three tissue cores (1.0 mm in diameter) from each neoplastic and normal tissue specimen. Cancers studied included breast (94 cases), prostate (38), lung (28), endometrium (27), colon (29), ovary (26), and melanoma (21). Normal tissues in the microarray were prostate (15), lung (6), endometrium (5), colon (4), ovary (2), and skin (3). Sections were immunostained, after prior pressure cooker antigen retrieval, using rabbit monoclonal AMACR antibody (1:40) (Zeta Corp, Sierra Madre, CA) and horseradish peroxidase-labeled polymer conjugated secondary antibody (Envision, Dako, Carpinteria, CA). A section of prostate cancer and prostatic intraepithelial neoplasia was used as positive control. Protein expression was scored as negative, weak (faint cytoplasmic or granular apical staining), moderate (diffuse granular cytoplasmic stain), and strong (diffuse intense cytoplasmic stain). Only moderate and strong staining was considered as positive staining, based on prior work. AMACR protein overexpression was found in several cancers, including prostate (34/38 [89.5%]), colon (13/29 [44.8%]), lung (4/28 [14.3%]), melanoma (2/21 [9.5%]), endometrium (2/27 [7.4%]), and breast (3/94 [3.2%]). None of the ovarian cancers (26 cases) demonstrated AMACR overexpression. AMACR expression was not present in any of the normal tissues nor in benign prostatic tissue associated with prostate carcinomas. This study suggests that AMACR is potentially an important tumor marker, particularly for prostate and colon cancer. It may be a useful adjunct to an immunohistochemical panel employed in the differential diagnosis of colon versus ovarian and breast carcinoma; the latter two infrequently express AMACR.

Original languageEnglish (US)
Pages (from-to)252-255
Number of pages4
JournalApplied Immunohistochemistry and Molecular Morphology
Volume13
Issue number3
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

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Racemases and Epimerases
Coenzyme A
Antibodies
Prostatic Neoplasms
Colon
Tumor Biomarkers
Endometrium
Prostatic Intraepithelial Neoplasia
Prostate
Neoplasms
Staining and Labeling
Lung
Ovary
Melanoma
Breast
Coloring Agents
Horseradish Peroxidase
Bile Acids and Salts
Ovarian Neoplasms
Colonic Neoplasms

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Medical Laboratory Technology

Cite this

Utility of α-methylacyl coenzyme A racemase (P504S antibody) as a diagnostic immunohistochemical marker for cancer. / Nassar, Aziza; Amin, Mahul; Sexton, Deborah G.; Cohen, Cynthia.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 13, No. 3, 01.09.2005, p. 252-255.

Research output: Contribution to journalArticle

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