Vaccination strategies to enhance local immunity and protection against Mycobacterium tuberculosis

Peter Klucar, Peter F. Barnes, Ying Kong, Susan T. Howard, Xiuhua Pang, Fang Fang Huang, Amy R. Tvinnereim, Buka Samten, Homayoun Shams

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To determine the immunogenicity and protective efficacy of the Mycobacterium tuberculosis 10 kD culture filtrate protein (CFP10), and to evaluate strategies that enhance local immunity, we used C57Bl/6 DR4 mice that were transgenic for human HLA DRB1*0401, because CFP10 contains epitopes for DRB1*0401 but not for C57Bl/6 mice. Intramuscular immunization with a DNA vaccine encoding CFP10 elicited production of IFN-γ by systemic CD4+ T cells, and one intravenous dose of the CFP10-based DNA vaccine coated with polyethylenimine (PEI) stimulated IFN-γ production by lung CD4+ cells and reduced the pulmonary bacillary burden. We conclude that CFP10 is a potential vaccine candidate and that coating vaccines with PEI enhances local protective immunity to tuberculosis.

Original languageEnglish (US)
Pages (from-to)1816-1824
Number of pages9
JournalVaccine
Volume27
Issue number12
DOIs
StatePublished - Mar 13 2009

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Polyethyleneimine
DNA Vaccines
Mycobacterium tuberculosis
recombinant vaccines
Immunity
Vaccination
Vaccines
immunity
lungs
vaccination
vaccines
Lung
mice
culture filtrates
tuberculosis
Transgenic Mice
epitopes
coatings
Epitopes
Immunization

All Science Journal Classification (ASJC) codes

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Klucar, P., Barnes, P. F., Kong, Y., Howard, S. T., Pang, X., Huang, F. F., ... Shams, H. (2009). Vaccination strategies to enhance local immunity and protection against Mycobacterium tuberculosis. Vaccine, 27(12), 1816-1824. https://doi.org/10.1016/j.vaccine.2009.01.119

Vaccination strategies to enhance local immunity and protection against Mycobacterium tuberculosis. / Klucar, Peter; Barnes, Peter F.; Kong, Ying; Howard, Susan T.; Pang, Xiuhua; Huang, Fang Fang; Tvinnereim, Amy R.; Samten, Buka; Shams, Homayoun.

In: Vaccine, Vol. 27, No. 12, 13.03.2009, p. 1816-1824.

Research output: Contribution to journalArticle

Klucar, P, Barnes, PF, Kong, Y, Howard, ST, Pang, X, Huang, FF, Tvinnereim, AR, Samten, B & Shams, H 2009, 'Vaccination strategies to enhance local immunity and protection against Mycobacterium tuberculosis', Vaccine, vol. 27, no. 12, pp. 1816-1824. https://doi.org/10.1016/j.vaccine.2009.01.119
Klucar, Peter ; Barnes, Peter F. ; Kong, Ying ; Howard, Susan T. ; Pang, Xiuhua ; Huang, Fang Fang ; Tvinnereim, Amy R. ; Samten, Buka ; Shams, Homayoun. / Vaccination strategies to enhance local immunity and protection against Mycobacterium tuberculosis. In: Vaccine. 2009 ; Vol. 27, No. 12. pp. 1816-1824.
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