Variable domain structure of κIV human light chain Len

High homology to the murine light chain McPC603

De Bin Huang, Chong Hwan Chang, Clint Ainsworth, George Johnson, Alan Solomon, Fred J. Stevens, Marianne Schiffer

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Antibody light chains of the κ subgroup are the predominant light chain component in human immune responses and are used almost exclusively in the antibody repertoire of mice. Human κ light chains comprise four subgroups. To date, all crystallographic studies of human κ light chains were carried out on proteins of the κI subgroup. The light chain produced by multiple myeloma patient Len, was of the κIV subgroup, it differed by only one residue from the germ-line gene encoded protein. The variable domain fragment of the light chain was crystallized from ammonium sulfate in space group C2221. The crystal structure was determined by molecular replacement and refined at 1.95 Å resolution to an R-factor of 0.15. Protein Len has six additional residues in its CDR1 segment compared to the κI proteins previously characterized. The κIV variable domain, Len, differs in only 23 of 113 residues from murine κ light chain McPC603. The RMS deviation upon superimposing their α-carbons was 0.69 Å. The CDR1 segment of the human and murine variable domains have the same length and conformation although their amino acid sequences differ in 5 out of 17 residues. Structural features were identified that could account for the significantly higher stability of the human κIV protein relative to its murine counterpart. This human κIV light chain structure is the closest human homolog to a murine light chain and can be expected to facilitate detailed structural comparisons necessary for effective humanization of murine antibodies.

Original languageEnglish (US)
Pages (from-to)1291-1301
Number of pages11
JournalMolecular Immunology
Volume34
Issue number18
DOIs
StatePublished - Dec 1 1997

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Light
Proteins
R388
Antibodies
Ammonium Sulfate
Multiple Myeloma
Germ Cells
Amino Acid Sequence
Carbon

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cite this

Variable domain structure of κIV human light chain Len : High homology to the murine light chain McPC603. / Huang, De Bin; Chang, Chong Hwan; Ainsworth, Clint; Johnson, George; Solomon, Alan; Stevens, Fred J.; Schiffer, Marianne.

In: Molecular Immunology, Vol. 34, No. 18, 01.12.1997, p. 1291-1301.

Research output: Contribution to journalArticle

Huang, De Bin ; Chang, Chong Hwan ; Ainsworth, Clint ; Johnson, George ; Solomon, Alan ; Stevens, Fred J. ; Schiffer, Marianne. / Variable domain structure of κIV human light chain Len : High homology to the murine light chain McPC603. In: Molecular Immunology. 1997 ; Vol. 34, No. 18. pp. 1291-1301.
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