Variable‐region subgroup distribution among λ‐type immunoglobulins in normal human serum

Masahiro Abe, Shuji Ozaki, Dennis Wolfenbarger, Mary Debram‐Hart, Deborah T. Weiss, Alan Solomon

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The distribution of the major VL subgroups (VλI, VλII, VλIII, VλIV, VλVI, and VλVIII) among λ‐type immunoglobulins (Igs) in normal serum was determined by a sandwich enzymelinked immunosorbent assay (ELISA) using a panel of murine anti‐human Vλ‐subgroup‐specific monoclonal antibodies (MoAbs) and appropriate reference standard proteins. The mean concentration of λI, λII, λIII, λIV, λVI, and λVIII Igs in serum specimens obtained from 23 adults was 2158, 162, 1958, 264, 225, and 169 μg/mL and represented 44, 3, 40, 5, 5, and 3% of the total Igλ population, respectively. The low percentage of λII Igs in normal serum was in marked contrast to the ∼40% incidence of this Vλ subgroup found among λ‐type Bence Jones proteins and monoclonal serum Igs obtained from patients with multiple myeloma and AL amyloidosis, or ∼60% in Waldenström's macroglobulinemia. The non‐random expresson of the VλII subgroup in these diseases implies a relationship between Vλ‐gene usage and plasma cell, as well as certain types of lymphocytic dyscrasias. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)4-9
Number of pages6
JournalJournal of Clinical Laboratory Analysis
Volume8
Issue number1
DOIs
StatePublished - 1994

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Immunoglobulins
Serum
Bence Jones Protein
Waldenstrom Macroglobulinemia
Immunosorbents
Amyloidosis
Plasma Cells
Multiple Myeloma
Assays
Monoclonal Antibodies
Plasmas
Incidence
Population
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

Cite this

Variable‐region subgroup distribution among λ‐type immunoglobulins in normal human serum. / Abe, Masahiro; Ozaki, Shuji; Wolfenbarger, Dennis; Debram‐Hart, Mary; Weiss, Deborah T.; Solomon, Alan.

In: Journal of Clinical Laboratory Analysis, Vol. 8, No. 1, 1994, p. 4-9.

Research output: Contribution to journalArticle

Abe, Masahiro ; Ozaki, Shuji ; Wolfenbarger, Dennis ; Debram‐Hart, Mary ; Weiss, Deborah T. ; Solomon, Alan. / Variable‐region subgroup distribution among λ‐type immunoglobulins in normal human serum. In: Journal of Clinical Laboratory Analysis. 1994 ; Vol. 8, No. 1. pp. 4-9.
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