Vascular endothelial cadherin (VE-cadherin)

Cloning and role in endothelial cell-cell adhesion

Jahanara Ali, Francesca-Fang Liao, Eric Martens, William A. Muller

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Objective: To identify proteins responsible for intercellular junction integrity in human umbilical vein endothelial cells (HUVEC). we produced a monoclonal antibody that recognized an endothelial cell-specific, junctionally restricted protein. We characterized and cloned the antigen to study its functional properties. Methods: The size and cellular distribution of the antigen were determined by immunofluorescence and immunoprecipitation. The molecule was cloned and transfected into cell lines, and its role in cell-cell adhesion and growth rate was determined. Results: Monoclonal antibody heel recognizes VE-cadherin, an endothelial cell-restricted cell adhesion molecule, VE-cadherin is localized to the borders between apposing endothelial cells but is diffusely distributed on subconfluent or migrating cells. Transfection of fibroblasts with VE-cadherin imparts to them the ability to adhere to each other in a calcium-dependent homophilic manner. Expression of VE-cadherin over a several-log range does not change the growth rate of these cells. Conclusions: Despite the fact that VE-cadherin is a "nonclassical" cadherin by structure. it functions as a classic cadherin by imparting to cells the ability to adhere in a calcium-dependent, homophilic manner. On HUVEC it appears to play a role in maintaining monolayer integrity.

Original languageEnglish (US)
Pages (from-to)267-277
Number of pages11
JournalMicrocirculation
Volume4
Issue number2
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Fingerprint

Cell Adhesion
Organism Cloning
Endothelial Cells
Human Umbilical Vein Endothelial Cells
Cadherins
Monoclonal Antibodies
Calcium
Antigens
Intercellular Junctions
Heel
Cell Adhesion Molecules
Growth
Immunoprecipitation
Fluorescent Antibody Technique
Transfection
Proteins
Fibroblasts
cadherin 5
Cell Line

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Vascular endothelial cadherin (VE-cadherin) : Cloning and role in endothelial cell-cell adhesion. / Ali, Jahanara; Liao, Francesca-Fang; Martens, Eric; Muller, William A.

In: Microcirculation, Vol. 4, No. 2, 01.01.1997, p. 267-277.

Research output: Contribution to journalArticle

Ali, Jahanara ; Liao, Francesca-Fang ; Martens, Eric ; Muller, William A. / Vascular endothelial cadherin (VE-cadherin) : Cloning and role in endothelial cell-cell adhesion. In: Microcirculation. 1997 ; Vol. 4, No. 2. pp. 267-277.
@article{4cbf66fb1ac14a6aad6115f2e30c78b3,
title = "Vascular endothelial cadherin (VE-cadherin): Cloning and role in endothelial cell-cell adhesion",
abstract = "Objective: To identify proteins responsible for intercellular junction integrity in human umbilical vein endothelial cells (HUVEC). we produced a monoclonal antibody that recognized an endothelial cell-specific, junctionally restricted protein. We characterized and cloned the antigen to study its functional properties. Methods: The size and cellular distribution of the antigen were determined by immunofluorescence and immunoprecipitation. The molecule was cloned and transfected into cell lines, and its role in cell-cell adhesion and growth rate was determined. Results: Monoclonal antibody heel recognizes VE-cadherin, an endothelial cell-restricted cell adhesion molecule, VE-cadherin is localized to the borders between apposing endothelial cells but is diffusely distributed on subconfluent or migrating cells. Transfection of fibroblasts with VE-cadherin imparts to them the ability to adhere to each other in a calcium-dependent homophilic manner. Expression of VE-cadherin over a several-log range does not change the growth rate of these cells. Conclusions: Despite the fact that VE-cadherin is a {"}nonclassical{"} cadherin by structure. it functions as a classic cadherin by imparting to cells the ability to adhere in a calcium-dependent, homophilic manner. On HUVEC it appears to play a role in maintaining monolayer integrity.",
author = "Jahanara Ali and Francesca-Fang Liao and Eric Martens and Muller, {William A.}",
year = "1997",
month = "1",
day = "1",
doi = "10.3109/10739689709146790",
language = "English (US)",
volume = "4",
pages = "267--277",
journal = "Microcirculation",
issn = "1073-9688",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Vascular endothelial cadherin (VE-cadherin)

T2 - Cloning and role in endothelial cell-cell adhesion

AU - Ali, Jahanara

AU - Liao, Francesca-Fang

AU - Martens, Eric

AU - Muller, William A.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Objective: To identify proteins responsible for intercellular junction integrity in human umbilical vein endothelial cells (HUVEC). we produced a monoclonal antibody that recognized an endothelial cell-specific, junctionally restricted protein. We characterized and cloned the antigen to study its functional properties. Methods: The size and cellular distribution of the antigen were determined by immunofluorescence and immunoprecipitation. The molecule was cloned and transfected into cell lines, and its role in cell-cell adhesion and growth rate was determined. Results: Monoclonal antibody heel recognizes VE-cadherin, an endothelial cell-restricted cell adhesion molecule, VE-cadherin is localized to the borders between apposing endothelial cells but is diffusely distributed on subconfluent or migrating cells. Transfection of fibroblasts with VE-cadherin imparts to them the ability to adhere to each other in a calcium-dependent homophilic manner. Expression of VE-cadherin over a several-log range does not change the growth rate of these cells. Conclusions: Despite the fact that VE-cadherin is a "nonclassical" cadherin by structure. it functions as a classic cadherin by imparting to cells the ability to adhere in a calcium-dependent, homophilic manner. On HUVEC it appears to play a role in maintaining monolayer integrity.

AB - Objective: To identify proteins responsible for intercellular junction integrity in human umbilical vein endothelial cells (HUVEC). we produced a monoclonal antibody that recognized an endothelial cell-specific, junctionally restricted protein. We characterized and cloned the antigen to study its functional properties. Methods: The size and cellular distribution of the antigen were determined by immunofluorescence and immunoprecipitation. The molecule was cloned and transfected into cell lines, and its role in cell-cell adhesion and growth rate was determined. Results: Monoclonal antibody heel recognizes VE-cadherin, an endothelial cell-restricted cell adhesion molecule, VE-cadherin is localized to the borders between apposing endothelial cells but is diffusely distributed on subconfluent or migrating cells. Transfection of fibroblasts with VE-cadherin imparts to them the ability to adhere to each other in a calcium-dependent homophilic manner. Expression of VE-cadherin over a several-log range does not change the growth rate of these cells. Conclusions: Despite the fact that VE-cadherin is a "nonclassical" cadherin by structure. it functions as a classic cadherin by imparting to cells the ability to adhere in a calcium-dependent, homophilic manner. On HUVEC it appears to play a role in maintaining monolayer integrity.

UR - http://www.scopus.com/inward/record.url?scp=0031156796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031156796&partnerID=8YFLogxK

U2 - 10.3109/10739689709146790

DO - 10.3109/10739689709146790

M3 - Article

VL - 4

SP - 267

EP - 277

JO - Microcirculation

JF - Microcirculation

SN - 1073-9688

IS - 2

ER -