Vascular Endothelial Growth Factor Isoform and Receptor Expression During Compensatory Lung Growth

Timothy Jancelewicz, Erich J. Grethel, Cheryl J. Chapin, Matthew S. Clifton, Kerilyn K. Nobuhara

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Vascular endothelial growth factor (VEGF) is required for blood vessel formation during lung growth and repair. Alteration of VEGF isoform expression has been demonstrated in response to fetal tracheal occlusion and in models of lung injury. The purpose of this study was to investigate VEGF expression during compensatory lung growth in the mouse. Methods: Under general anesthesia, adult mice underwent left thoracotomy with (n = 5) or without (sham, n = 5) pneumonectomy. The right lungs were harvested at 1, 3, and 7 d after the operation. Lung-to-body weight ratio as well as total DNA and protein content were measured. VEGF protein expression was analyzed by Western blot and ELISA. VEGF isoform expression was evaluated using semi-quantitative PCR followed by Imagequant optical densitometry. Values were compared by Student's t-test and ANOVA using Fisher's protected least significant difference post-hoc test where appropriate. Results: Compensatory lung growth was observed as measured by increases in right lung-to-body weight ratio and in DNA and protein content. Total VEGF RNA and protein expression did not change after pneumonectomy. However, on post-operative day 1, there was a decrease in the relative percentage of VEGF188 mRNA (P < 0.01), and an increase in the relative percentage of VEGF164 mRNA (P = 0.05). At 3 d postpneumonectomy, low relative VEGF188 expression persisted (P < 0.05), VEGF164 expression normalized, and relative VEGF120 expression increased (P < 0.01). Isoform expression in the pneumonectomy animals was identical to sham animals by the seventh d. There were no differences observed in VEGF receptor expression. Conclusion: During compensatory lung growth, we have observed an early postoperative reversion of VEGF isoform expression to the pattern seen during fetal lung development and in lung injury models.

Original languageEnglish (US)
Pages (from-to)107-113
Number of pages7
JournalJournal of Surgical Research
Volume160
Issue number1
DOIs
StatePublished - May 1 2010
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factor Receptor
Protein Isoforms
Vascular Endothelial Growth Factor A
Lung
Growth
Pneumonectomy
Lung Injury
Proteins
Body Weight
Messenger RNA
Densitometry
DNA
Thoracotomy
Fetal Development
General Anesthesia
Blood Vessels
Analysis of Variance
Western Blotting
Enzyme-Linked Immunosorbent Assay
RNA

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Vascular Endothelial Growth Factor Isoform and Receptor Expression During Compensatory Lung Growth. / Jancelewicz, Timothy; Grethel, Erich J.; Chapin, Cheryl J.; Clifton, Matthew S.; Nobuhara, Kerilyn K.

In: Journal of Surgical Research, Vol. 160, No. 1, 01.05.2010, p. 107-113.

Research output: Contribution to journalArticle

Jancelewicz, Timothy ; Grethel, Erich J. ; Chapin, Cheryl J. ; Clifton, Matthew S. ; Nobuhara, Kerilyn K. / Vascular Endothelial Growth Factor Isoform and Receptor Expression During Compensatory Lung Growth. In: Journal of Surgical Research. 2010 ; Vol. 160, No. 1. pp. 107-113.
@article{c405ea5fce4445fb9fd0820bdb21973f,
title = "Vascular Endothelial Growth Factor Isoform and Receptor Expression During Compensatory Lung Growth",
abstract = "Background: Vascular endothelial growth factor (VEGF) is required for blood vessel formation during lung growth and repair. Alteration of VEGF isoform expression has been demonstrated in response to fetal tracheal occlusion and in models of lung injury. The purpose of this study was to investigate VEGF expression during compensatory lung growth in the mouse. Methods: Under general anesthesia, adult mice underwent left thoracotomy with (n = 5) or without (sham, n = 5) pneumonectomy. The right lungs were harvested at 1, 3, and 7 d after the operation. Lung-to-body weight ratio as well as total DNA and protein content were measured. VEGF protein expression was analyzed by Western blot and ELISA. VEGF isoform expression was evaluated using semi-quantitative PCR followed by Imagequant optical densitometry. Values were compared by Student's t-test and ANOVA using Fisher's protected least significant difference post-hoc test where appropriate. Results: Compensatory lung growth was observed as measured by increases in right lung-to-body weight ratio and in DNA and protein content. Total VEGF RNA and protein expression did not change after pneumonectomy. However, on post-operative day 1, there was a decrease in the relative percentage of VEGF188 mRNA (P < 0.01), and an increase in the relative percentage of VEGF164 mRNA (P = 0.05). At 3 d postpneumonectomy, low relative VEGF188 expression persisted (P < 0.05), VEGF164 expression normalized, and relative VEGF120 expression increased (P < 0.01). Isoform expression in the pneumonectomy animals was identical to sham animals by the seventh d. There were no differences observed in VEGF receptor expression. Conclusion: During compensatory lung growth, we have observed an early postoperative reversion of VEGF isoform expression to the pattern seen during fetal lung development and in lung injury models.",
author = "Timothy Jancelewicz and Grethel, {Erich J.} and Chapin, {Cheryl J.} and Clifton, {Matthew S.} and Nobuhara, {Kerilyn K.}",
year = "2010",
month = "5",
day = "1",
doi = "10.1016/j.jss.2008.10.007",
language = "English (US)",
volume = "160",
pages = "107--113",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Vascular Endothelial Growth Factor Isoform and Receptor Expression During Compensatory Lung Growth

AU - Jancelewicz, Timothy

AU - Grethel, Erich J.

AU - Chapin, Cheryl J.

AU - Clifton, Matthew S.

AU - Nobuhara, Kerilyn K.

PY - 2010/5/1

Y1 - 2010/5/1

N2 - Background: Vascular endothelial growth factor (VEGF) is required for blood vessel formation during lung growth and repair. Alteration of VEGF isoform expression has been demonstrated in response to fetal tracheal occlusion and in models of lung injury. The purpose of this study was to investigate VEGF expression during compensatory lung growth in the mouse. Methods: Under general anesthesia, adult mice underwent left thoracotomy with (n = 5) or without (sham, n = 5) pneumonectomy. The right lungs were harvested at 1, 3, and 7 d after the operation. Lung-to-body weight ratio as well as total DNA and protein content were measured. VEGF protein expression was analyzed by Western blot and ELISA. VEGF isoform expression was evaluated using semi-quantitative PCR followed by Imagequant optical densitometry. Values were compared by Student's t-test and ANOVA using Fisher's protected least significant difference post-hoc test where appropriate. Results: Compensatory lung growth was observed as measured by increases in right lung-to-body weight ratio and in DNA and protein content. Total VEGF RNA and protein expression did not change after pneumonectomy. However, on post-operative day 1, there was a decrease in the relative percentage of VEGF188 mRNA (P < 0.01), and an increase in the relative percentage of VEGF164 mRNA (P = 0.05). At 3 d postpneumonectomy, low relative VEGF188 expression persisted (P < 0.05), VEGF164 expression normalized, and relative VEGF120 expression increased (P < 0.01). Isoform expression in the pneumonectomy animals was identical to sham animals by the seventh d. There were no differences observed in VEGF receptor expression. Conclusion: During compensatory lung growth, we have observed an early postoperative reversion of VEGF isoform expression to the pattern seen during fetal lung development and in lung injury models.

AB - Background: Vascular endothelial growth factor (VEGF) is required for blood vessel formation during lung growth and repair. Alteration of VEGF isoform expression has been demonstrated in response to fetal tracheal occlusion and in models of lung injury. The purpose of this study was to investigate VEGF expression during compensatory lung growth in the mouse. Methods: Under general anesthesia, adult mice underwent left thoracotomy with (n = 5) or without (sham, n = 5) pneumonectomy. The right lungs were harvested at 1, 3, and 7 d after the operation. Lung-to-body weight ratio as well as total DNA and protein content were measured. VEGF protein expression was analyzed by Western blot and ELISA. VEGF isoform expression was evaluated using semi-quantitative PCR followed by Imagequant optical densitometry. Values were compared by Student's t-test and ANOVA using Fisher's protected least significant difference post-hoc test where appropriate. Results: Compensatory lung growth was observed as measured by increases in right lung-to-body weight ratio and in DNA and protein content. Total VEGF RNA and protein expression did not change after pneumonectomy. However, on post-operative day 1, there was a decrease in the relative percentage of VEGF188 mRNA (P < 0.01), and an increase in the relative percentage of VEGF164 mRNA (P = 0.05). At 3 d postpneumonectomy, low relative VEGF188 expression persisted (P < 0.05), VEGF164 expression normalized, and relative VEGF120 expression increased (P < 0.01). Isoform expression in the pneumonectomy animals was identical to sham animals by the seventh d. There were no differences observed in VEGF receptor expression. Conclusion: During compensatory lung growth, we have observed an early postoperative reversion of VEGF isoform expression to the pattern seen during fetal lung development and in lung injury models.

UR - http://www.scopus.com/inward/record.url?scp=77950189435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950189435&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2008.10.007

DO - 10.1016/j.jss.2008.10.007

M3 - Article

VL - 160

SP - 107

EP - 113

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 1

ER -