Vascular endothelial growth factor, placenta growth factor and their receptors in isolated human trophoblast

V. H. Shore, T. H. Wang, C. L. Wang, R. J. Torry, Michael Caudle, D. S. Torry

Research output: Contribution to journalArticle

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Abstract

The expression of the angiogenic growth factors, vascular endothelial cell growth factor (VEGF) and placenta growth factor (PlGF) was demonstrated in isolated human term cytotrophoblast and in vitro differentiated syncytiotrophoblast. RNase protection assays demonstrated VEGF expression in both cytotrophoblast and syncytiotrophoblast while prominent PlGF expression was detected in both types of trophoblast by Northern blot analyses. VEGF expression increased approximately eightfold in trophoblast cultured under hypoxic conditions (1 per cent O 2 ) yet PlGF expression decreased 73 ± 5.5 per cent in the same trophoblast. These results suggest distinct regulatory mechanisms govern expression of VEGF and PlGF in trophoblast. Characterization of the VEGF/PlGF receptors, KDR and flt-1, revealed the presence of flt-1 mRNA in isolated cytotrophoblast and in vitro differentiated syncytiotrophoblast. KDR was not detected in the isolated trophoblast. Exogenous rhVEGF induced c-Jun N-terminal kinase (JNK) activity in the normal trophoblast indicating that the flt-1 receptors on trophoblast are functional. Trophoblast-derived VEGF/PlGF could act in a paracrine fashion to promote uterine angiogenesis and vascular permeability within the placental bed. In addition, presence of functional flt-1 on normal trophoblast suggests that VEGF/PlGF functions in an autocrine manner to perform an as yet undefined role in trophoblast invasion, differentiation, and/or metabolic activity during placentation.

Original languageEnglish (US)
Pages (from-to)657-665
Number of pages9
JournalPlacenta
Volume18
Issue number8
DOIs
StatePublished - Jan 1 1997

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Growth Factor Receptors
Trophoblasts
Vascular Endothelial Growth Factor A
Placenta Growth Factor
Placentation
JNK Mitogen-Activated Protein Kinases
Angiogenesis Inducing Agents
Capillary Permeability
Ribonucleases
Northern Blotting

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Vascular endothelial growth factor, placenta growth factor and their receptors in isolated human trophoblast. / Shore, V. H.; Wang, T. H.; Wang, C. L.; Torry, R. J.; Caudle, Michael; Torry, D. S.

In: Placenta, Vol. 18, No. 8, 01.01.1997, p. 657-665.

Research output: Contribution to journalArticle

Shore, V. H. ; Wang, T. H. ; Wang, C. L. ; Torry, R. J. ; Caudle, Michael ; Torry, D. S. / Vascular endothelial growth factor, placenta growth factor and their receptors in isolated human trophoblast. In: Placenta. 1997 ; Vol. 18, No. 8. pp. 657-665.
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