Vascular responses to vasopressin are tone-dependent in the cerebral circulation of the newborn pig

W. M. Armstead, R. Mirro, D. W. Busija, Charles Leffler

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The effects of lysine vasopressin (LVP) on pial arteriolar diameter and cortical periarachnoid fluid prostanoid concentrations were investigated in newborn pigs. Chloralose-anesthetized piglets were equipped with closed cranial windows over the parietal cortex for observation of pial arterioles and collection of cerebrospinal fluid (CSF) passing over the cerebral surface. Prostanoids in the CSF were determined by radioimmunoassay. LVP (10-1,000 μU/ml) elicited concentration-dependent increases in pial arteriolar diameter associated with increased levels of 6-keto-prostaglandin (PG)F(1α), PGE2, thromboxane B2, and PGF(2α). LVP-induced pial arteriolar dilation was unchanged after intravenous indomethacin (5 mg/kg). Conversely, LVP constricts pial arterioles previously dilated by physiological (hemorrhagic hypotension) and pharmacological (topically applied PGE2 or isoproterenol) intervention. This constriction is potentiated by indomethacin. Vascular and biochemical changes elicited by LVP were blocked by intravenous [1-(β-mercapto-ββ-cyclopentamethylene propionic acid),2,(O-methyl)-Tyr-AVP] (5 μg/kg), a putative V1 receptor antagonist, whereas vascular effects of norepinephrine and U46619, a thromboxane A2 mimic, were unchanged. Therefore, the degree of vascular tone appears to influence responses of the newborn pig cerebral circulation to LVP.

Original languageEnglish (US)
Pages (from-to)136-144
Number of pages9
JournalCirculation research
Volume64
Issue number1
DOIs
StatePublished - Jan 1 1989

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Cerebrovascular Circulation
Lypressin
Vasopressins
Blood Vessels
Swine
Arterioles
Prostaglandins F
Dinoprostone
Indomethacin
Prostaglandins
Cerebrospinal Fluid
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Vasopressin Receptors
Thromboxane B2
Chloralose
Parietal Lobe
Thromboxane A2
Isoproterenol
Constriction
Hypotension

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Vascular responses to vasopressin are tone-dependent in the cerebral circulation of the newborn pig. / Armstead, W. M.; Mirro, R.; Busija, D. W.; Leffler, Charles.

In: Circulation research, Vol. 64, No. 1, 01.01.1989, p. 136-144.

Research output: Contribution to journalArticle

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