Vasodilation effect of nitrate FLNT on the porcine coronary artery and its mechanism

Zhan Qing Wang, Kai Chen, Jin Ping Wang, Nan Jiang, Ming Zeng, Jing Feng Xu

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the vasodilation effect on the porcine coronary artery and the possible mechanism of nitrate FLNT. Methods: The tension of porcine coronary artery precontracted with potassium chloride (KCl, 30 mmol · L -1 ), histamine (His, 10 -3 mmol · L -1 ), or acetylcholine (ACh, 10 -3 mmol · L -1 ) was measured. The vasodilation effect of FLNT was compared to that of nitroglycerin (NG) or isosorbide dinitrate (ISDN). Mythelene blue, the blocker of guanylate cyclase (GC), and the specific blockers of inward rectifier potassium channel, Ca 2+ activated potassium channel, voltage-dependent potassium channel and ATP-sensitive potassium channel (BaCl 2 , tetraethylammonium, 4-aminopyridine, glibenclamide) were used, respectively, to clarify the mechanism of FLNT vasodilation. Results: In porcine coronary artery rings precontracted with KCl, His or ACh, FLNT (10 -4 - 10 -1 mmol · L -1 ) caused the concentration-dependent relaxation in both the endothelium intact and denuded arterial rings. FLNT relaxation was almost the same as ISDN. FLNT shifted KCl concentration-response curve to the right and decreased the maximum response with a pD′ 2 of 3.68. Mythelene blue reduced FLNT vasodilatin. FLNT, like nicorandil, reduced the vessel tension precontracted with some potassium channel blockers. Conclusion: FLNT endothelium-independently and concentration-dependently induces relaxation of porcine coronary artery, which is similar to isosorbide dinitrate. The mechanism may relate to guanylate cyclase activation, potassium channel opening, and the inhibition of voltage-dependent Ca 2+ channel and receptor-controlled Ca 2+ channel.

Original languageEnglish (US)
JournalChinese Journal of New Drugs
Volume17
Issue number8
StatePublished - May 23 2008
Externally publishedYes

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Isosorbide Dinitrate
Vasodilation
Nitrates
Potassium Channels
Coronary Vessels
Swine
Guanylate Cyclase
Endothelium
Potassium Channel Blockers
Nicorandil
KATP Channels
4-Aminopyridine
Tetraethylammonium
Potassium Chloride
Glyburide
Nitroglycerin
Histamine
Acetylcholine

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Cite this

Vasodilation effect of nitrate FLNT on the porcine coronary artery and its mechanism. / Wang, Zhan Qing; Chen, Kai; Wang, Jin Ping; Jiang, Nan; Zeng, Ming; Xu, Jing Feng.

In: Chinese Journal of New Drugs, Vol. 17, No. 8, 23.05.2008.

Research output: Contribution to journalArticle

Wang, Zhan Qing ; Chen, Kai ; Wang, Jin Ping ; Jiang, Nan ; Zeng, Ming ; Xu, Jing Feng. / Vasodilation effect of nitrate FLNT on the porcine coronary artery and its mechanism. In: Chinese Journal of New Drugs. 2008 ; Vol. 17, No. 8.
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AB - Objective: To investigate the vasodilation effect on the porcine coronary artery and the possible mechanism of nitrate FLNT. Methods: The tension of porcine coronary artery precontracted with potassium chloride (KCl, 30 mmol · L -1 ), histamine (His, 10 -3 mmol · L -1 ), or acetylcholine (ACh, 10 -3 mmol · L -1 ) was measured. The vasodilation effect of FLNT was compared to that of nitroglycerin (NG) or isosorbide dinitrate (ISDN). Mythelene blue, the blocker of guanylate cyclase (GC), and the specific blockers of inward rectifier potassium channel, Ca 2+ activated potassium channel, voltage-dependent potassium channel and ATP-sensitive potassium channel (BaCl 2 , tetraethylammonium, 4-aminopyridine, glibenclamide) were used, respectively, to clarify the mechanism of FLNT vasodilation. Results: In porcine coronary artery rings precontracted with KCl, His or ACh, FLNT (10 -4 - 10 -1 mmol · L -1 ) caused the concentration-dependent relaxation in both the endothelium intact and denuded arterial rings. FLNT relaxation was almost the same as ISDN. FLNT shifted KCl concentration-response curve to the right and decreased the maximum response with a pD′ 2 of 3.68. Mythelene blue reduced FLNT vasodilatin. FLNT, like nicorandil, reduced the vessel tension precontracted with some potassium channel blockers. Conclusion: FLNT endothelium-independently and concentration-dependently induces relaxation of porcine coronary artery, which is similar to isosorbide dinitrate. The mechanism may relate to guanylate cyclase activation, potassium channel opening, and the inhibition of voltage-dependent Ca 2+ channel and receptor-controlled Ca 2+ channel.

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