Voltage-gated potassium currents in acutely dissociated rat cortical neurons

Robert Foehring, D. J. Surmeier

Research output: Contribution to journalArticle

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Abstract

1. We describe three outward K+ current components in acutely dissociated neurons from rat sensorimotor cortex on the basis of inactivation kinetics and voltage dependence. 2. The fast A current (I(Af)) was completely inactivated at -40 mV and half-inactivated at -52 mV. It activated [time to peak (TTP) 8 ms at -10 mV] and was inactivated (τ(inact) = 12 ms at -10 mV) rapidly. Recovery from inactivation had a time constant of ~80 ms at -100 mV. It was insensitive to tetraethyl ammonium (TEA) and dendrotoxin but was blocked by 4-aminopyridine (4-AP, IC50 = 1 mM). 3. The slowly inactivating current (I(KS)) was the largest current seen in acutely dissociated adult neurons. It was completely inactivated at -40 mV, half-inactivated at -98 mV, and was kinetically slower (TTP = 130 ms at -10 mV; τ(inact) = 293 ms at - 10 mV) than the fast A current. Deactivation tails were fit with the sum of two exponentials with time constants of 2-10 and 15-40 ms. I(KS) recovered from inactivation with a time constant of ~1,200 ms at -100 mV. 4. There were two components that inactivated with even slower kinetics. The very slowly inactivating current (I(KSS)) was operationally defined as the current remaining after a 5-s hold at -40 mV. One component inactivated with a time constant of 1,927 ms at -10 mV. The other component showed no inactivation over a 5-s test command, but in 40- to 50-s steps to -10 mV, inactivated with a τ of ~20s. The very slowly inactivating current activated with similar kinetics to I(KS) (TTP = 121 ms at -10 mV), and two deactivation tails, with kinetics similar to those after the -100 mV prepulse, were observed after holding at -40 mV. 5. Both I(KS) and I(KSS) were sensitive to TEA. Seventy- six percent (76%) of I(KSS) was blocked by 30 mM TEA. Two components to the TEA block were present for I(KSS), with IC50s of 88 μM (67% of blockable current) and 7 mM (33%). Seventy percent (70%) of I(KS) was blocked by 30 mM TEA. For the I(KS) current, there were also two effective concentrations, with IC50s of 8 μM (21% of blockable current) and 3 mM (79%). 6. I(KS) and I(KSS) were also sensitive to 4-AP. Seventy-six percent (76%) of I(KSS) was blocked by 3-5 mM 4-AP. I(KSS) exhibited two components of 4-AP block. The high-affinity component had an IC50 of 90 μM (66% of blockable current), and the low-affinity component had an IC50 of 7 mM (34% of current). Only 24% of I(KS) was sensitive to 3-5 mM 4-AP. This block was well fit by a single IC50 of 92 μM. 7. I(KS) and I(KSS) were also dendrotoxin sensitive. I(KSS) was blocked by 100 nM dendrotoxin (100% block). I(KSS) exhibited two components of dendrotoxin block, with IC50s of 266 pM (46% of current) and 27 nM (54%). Only 36% of I(KS) was blocked by 100 nM dendrotoxin. This block had two components with IC50s of 146 pM (29% of blockable current) and 10.4 nM (71% of current). 8. A model is discussed in which the voltage-gated K+ currents are represented by four channel types: fast A (A(f)), slowly inactivating (K1), and two very slowly inactivating types (K2 and K3).

Original languageEnglish (US)
Pages (from-to)51-63
Number of pages13
JournalJournal of neurophysiology
Volume70
Issue number1
DOIs
StatePublished - Jan 1 1993

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Potassium
Neurons
Ammonium Compounds
Inhibitory Concentration 50
KS I
4-Aminopyridine
Transcription Factor AP-1
Tail
dendrotoxin

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

Cite this

Voltage-gated potassium currents in acutely dissociated rat cortical neurons. / Foehring, Robert; Surmeier, D. J.

In: Journal of neurophysiology, Vol. 70, No. 1, 01.01.1993, p. 51-63.

Research output: Contribution to journalArticle

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abstract = "1. We describe three outward K+ current components in acutely dissociated neurons from rat sensorimotor cortex on the basis of inactivation kinetics and voltage dependence. 2. The fast A current (I(Af)) was completely inactivated at -40 mV and half-inactivated at -52 mV. It activated [time to peak (TTP) 8 ms at -10 mV] and was inactivated (τ(inact) = 12 ms at -10 mV) rapidly. Recovery from inactivation had a time constant of ~80 ms at -100 mV. It was insensitive to tetraethyl ammonium (TEA) and dendrotoxin but was blocked by 4-aminopyridine (4-AP, IC50 = 1 mM). 3. The slowly inactivating current (I(KS)) was the largest current seen in acutely dissociated adult neurons. It was completely inactivated at -40 mV, half-inactivated at -98 mV, and was kinetically slower (TTP = 130 ms at -10 mV; τ(inact) = 293 ms at - 10 mV) than the fast A current. Deactivation tails were fit with the sum of two exponentials with time constants of 2-10 and 15-40 ms. I(KS) recovered from inactivation with a time constant of ~1,200 ms at -100 mV. 4. There were two components that inactivated with even slower kinetics. The very slowly inactivating current (I(KSS)) was operationally defined as the current remaining after a 5-s hold at -40 mV. One component inactivated with a time constant of 1,927 ms at -10 mV. The other component showed no inactivation over a 5-s test command, but in 40- to 50-s steps to -10 mV, inactivated with a τ of ~20s. The very slowly inactivating current activated with similar kinetics to I(KS) (TTP = 121 ms at -10 mV), and two deactivation tails, with kinetics similar to those after the -100 mV prepulse, were observed after holding at -40 mV. 5. Both I(KS) and I(KSS) were sensitive to TEA. Seventy- six percent (76{\%}) of I(KSS) was blocked by 30 mM TEA. Two components to the TEA block were present for I(KSS), with IC50s of 88 μM (67{\%} of blockable current) and 7 mM (33{\%}). Seventy percent (70{\%}) of I(KS) was blocked by 30 mM TEA. For the I(KS) current, there were also two effective concentrations, with IC50s of 8 μM (21{\%} of blockable current) and 3 mM (79{\%}). 6. I(KS) and I(KSS) were also sensitive to 4-AP. Seventy-six percent (76{\%}) of I(KSS) was blocked by 3-5 mM 4-AP. I(KSS) exhibited two components of 4-AP block. The high-affinity component had an IC50 of 90 μM (66{\%} of blockable current), and the low-affinity component had an IC50 of 7 mM (34{\%} of current). Only 24{\%} of I(KS) was sensitive to 3-5 mM 4-AP. This block was well fit by a single IC50 of 92 μM. 7. I(KS) and I(KSS) were also dendrotoxin sensitive. I(KSS) was blocked by 100 nM dendrotoxin (100{\%} block). I(KSS) exhibited two components of dendrotoxin block, with IC50s of 266 pM (46{\%} of current) and 27 nM (54{\%}). Only 36{\%} of I(KS) was blocked by 100 nM dendrotoxin. This block had two components with IC50s of 146 pM (29{\%} of blockable current) and 10.4 nM (71{\%} of current). 8. A model is discussed in which the voltage-gated K+ currents are represented by four channel types: fast A (A(f)), slowly inactivating (K1), and two very slowly inactivating types (K2 and K3).",
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N2 - 1. We describe three outward K+ current components in acutely dissociated neurons from rat sensorimotor cortex on the basis of inactivation kinetics and voltage dependence. 2. The fast A current (I(Af)) was completely inactivated at -40 mV and half-inactivated at -52 mV. It activated [time to peak (TTP) 8 ms at -10 mV] and was inactivated (τ(inact) = 12 ms at -10 mV) rapidly. Recovery from inactivation had a time constant of ~80 ms at -100 mV. It was insensitive to tetraethyl ammonium (TEA) and dendrotoxin but was blocked by 4-aminopyridine (4-AP, IC50 = 1 mM). 3. The slowly inactivating current (I(KS)) was the largest current seen in acutely dissociated adult neurons. It was completely inactivated at -40 mV, half-inactivated at -98 mV, and was kinetically slower (TTP = 130 ms at -10 mV; τ(inact) = 293 ms at - 10 mV) than the fast A current. Deactivation tails were fit with the sum of two exponentials with time constants of 2-10 and 15-40 ms. I(KS) recovered from inactivation with a time constant of ~1,200 ms at -100 mV. 4. There were two components that inactivated with even slower kinetics. The very slowly inactivating current (I(KSS)) was operationally defined as the current remaining after a 5-s hold at -40 mV. One component inactivated with a time constant of 1,927 ms at -10 mV. The other component showed no inactivation over a 5-s test command, but in 40- to 50-s steps to -10 mV, inactivated with a τ of ~20s. The very slowly inactivating current activated with similar kinetics to I(KS) (TTP = 121 ms at -10 mV), and two deactivation tails, with kinetics similar to those after the -100 mV prepulse, were observed after holding at -40 mV. 5. Both I(KS) and I(KSS) were sensitive to TEA. Seventy- six percent (76%) of I(KSS) was blocked by 30 mM TEA. Two components to the TEA block were present for I(KSS), with IC50s of 88 μM (67% of blockable current) and 7 mM (33%). Seventy percent (70%) of I(KS) was blocked by 30 mM TEA. For the I(KS) current, there were also two effective concentrations, with IC50s of 8 μM (21% of blockable current) and 3 mM (79%). 6. I(KS) and I(KSS) were also sensitive to 4-AP. Seventy-six percent (76%) of I(KSS) was blocked by 3-5 mM 4-AP. I(KSS) exhibited two components of 4-AP block. The high-affinity component had an IC50 of 90 μM (66% of blockable current), and the low-affinity component had an IC50 of 7 mM (34% of current). Only 24% of I(KS) was sensitive to 3-5 mM 4-AP. This block was well fit by a single IC50 of 92 μM. 7. I(KS) and I(KSS) were also dendrotoxin sensitive. I(KSS) was blocked by 100 nM dendrotoxin (100% block). I(KSS) exhibited two components of dendrotoxin block, with IC50s of 266 pM (46% of current) and 27 nM (54%). Only 36% of I(KS) was blocked by 100 nM dendrotoxin. This block had two components with IC50s of 146 pM (29% of blockable current) and 10.4 nM (71% of current). 8. A model is discussed in which the voltage-gated K+ currents are represented by four channel types: fast A (A(f)), slowly inactivating (K1), and two very slowly inactivating types (K2 and K3).

AB - 1. We describe three outward K+ current components in acutely dissociated neurons from rat sensorimotor cortex on the basis of inactivation kinetics and voltage dependence. 2. The fast A current (I(Af)) was completely inactivated at -40 mV and half-inactivated at -52 mV. It activated [time to peak (TTP) 8 ms at -10 mV] and was inactivated (τ(inact) = 12 ms at -10 mV) rapidly. Recovery from inactivation had a time constant of ~80 ms at -100 mV. It was insensitive to tetraethyl ammonium (TEA) and dendrotoxin but was blocked by 4-aminopyridine (4-AP, IC50 = 1 mM). 3. The slowly inactivating current (I(KS)) was the largest current seen in acutely dissociated adult neurons. It was completely inactivated at -40 mV, half-inactivated at -98 mV, and was kinetically slower (TTP = 130 ms at -10 mV; τ(inact) = 293 ms at - 10 mV) than the fast A current. Deactivation tails were fit with the sum of two exponentials with time constants of 2-10 and 15-40 ms. I(KS) recovered from inactivation with a time constant of ~1,200 ms at -100 mV. 4. There were two components that inactivated with even slower kinetics. The very slowly inactivating current (I(KSS)) was operationally defined as the current remaining after a 5-s hold at -40 mV. One component inactivated with a time constant of 1,927 ms at -10 mV. The other component showed no inactivation over a 5-s test command, but in 40- to 50-s steps to -10 mV, inactivated with a τ of ~20s. The very slowly inactivating current activated with similar kinetics to I(KS) (TTP = 121 ms at -10 mV), and two deactivation tails, with kinetics similar to those after the -100 mV prepulse, were observed after holding at -40 mV. 5. Both I(KS) and I(KSS) were sensitive to TEA. Seventy- six percent (76%) of I(KSS) was blocked by 30 mM TEA. Two components to the TEA block were present for I(KSS), with IC50s of 88 μM (67% of blockable current) and 7 mM (33%). Seventy percent (70%) of I(KS) was blocked by 30 mM TEA. For the I(KS) current, there were also two effective concentrations, with IC50s of 8 μM (21% of blockable current) and 3 mM (79%). 6. I(KS) and I(KSS) were also sensitive to 4-AP. Seventy-six percent (76%) of I(KSS) was blocked by 3-5 mM 4-AP. I(KSS) exhibited two components of 4-AP block. The high-affinity component had an IC50 of 90 μM (66% of blockable current), and the low-affinity component had an IC50 of 7 mM (34% of current). Only 24% of I(KS) was sensitive to 3-5 mM 4-AP. This block was well fit by a single IC50 of 92 μM. 7. I(KS) and I(KSS) were also dendrotoxin sensitive. I(KSS) was blocked by 100 nM dendrotoxin (100% block). I(KSS) exhibited two components of dendrotoxin block, with IC50s of 266 pM (46% of current) and 27 nM (54%). Only 36% of I(KS) was blocked by 100 nM dendrotoxin. This block had two components with IC50s of 146 pM (29% of blockable current) and 10.4 nM (71% of current). 8. A model is discussed in which the voltage-gated K+ currents are represented by four channel types: fast A (A(f)), slowly inactivating (K1), and two very slowly inactivating types (K2 and K3).

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