WASP - mice exhibit defective immune responses to influenza A virus, Streptococcus pneumoniae, and Mycobacterium bovis BCG

Samita Andreansky, Haiyan Liu, Stephen Turner, Jonathan Mccullers, Roland Lang, Robert Rutschman, Peter C. Doherty, Peter J. Murray, Arthur W. Nienhuis, Ted S. Strom

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objective. To quantify the immune response of WASP - mice to three different pathogens: influenza A virus, Streptococcus pneumoniae, and Mycobacterium bovis. Methods. Primary and secondary T-cell responses to influenza A virus were quantified via tetramer assays. Viral clearance from lung was also measured. Lethality of intranasal inoculation with luminescent S. pneumoniae was assessed by dose escalation and direct luminescence imaging. After intravenous inoculation with M. bovis, residual mycobacteria in lung, liver, and spleen were measured by standard culture methods. Results. The reduced secondary T-cell response to influenza A virus correlates with a relative but not absolute loss of splenic T and B cells similar to that seen in clinical Wiskott-Aldrich Syndrome (WAS), and slower clearance of virus from lung. The reduced magnitude of the secondary T-cell response correlates with a progressive loss of influenza-specific T cells after primary inoculation. WASP - mice show an increased susceptibility to lethal pneumonia after intranasal inoculation with S. pneumoniae, which is among the most frequent causes of clinical complications in WAS patients. WASP - mice clear M. bovis bacille Calmette-Guerin (BCG) more slowly from lung, liver, and spleen. Bone marrow-derived macrophages, however, show normal ex vivo cytokine secretion in response to M. bovis. Conclusions. These results demonstrate that WASP - mice are functionally immunodeficient in regard to three different pathogens, and provide relevant end points for the study of treatment modalities in this model. They also suggest a specific physiologic mechanism, failure to accumulate memory T cells, for at least one of the defective immune responses.

Original languageEnglish (US)
Pages (from-to)443-451
Number of pages9
JournalExperimental Hematology
Volume33
Issue number4
DOIs
StatePublished - Jan 1 2005

Fingerprint

Streptococcus bovis
Influenza A virus
Mycobacterium bovis
Streptococcus pneumoniae
T-Lymphocytes
Wiskott-Aldrich Syndrome
Lung
Spleen
Liver
Mycobacterium
Luminescence
Human Influenza
Pneumonia
B-Lymphocytes
Macrophages
Cytokines
Viruses

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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WASP - mice exhibit defective immune responses to influenza A virus, Streptococcus pneumoniae, and Mycobacterium bovis BCG . / Andreansky, Samita; Liu, Haiyan; Turner, Stephen; Mccullers, Jonathan; Lang, Roland; Rutschman, Robert; Doherty, Peter C.; Murray, Peter J.; Nienhuis, Arthur W.; Strom, Ted S.

In: Experimental Hematology, Vol. 33, No. 4, 01.01.2005, p. 443-451.

Research output: Contribution to journalArticle

Andreansky, S, Liu, H, Turner, S, Mccullers, J, Lang, R, Rutschman, R, Doherty, PC, Murray, PJ, Nienhuis, AW & Strom, TS 2005, ' WASP - mice exhibit defective immune responses to influenza A virus, Streptococcus pneumoniae, and Mycobacterium bovis BCG ', Experimental Hematology, vol. 33, no. 4, pp. 443-451. https://doi.org/10.1016/j.exphem.2004.12.006
Andreansky, Samita ; Liu, Haiyan ; Turner, Stephen ; Mccullers, Jonathan ; Lang, Roland ; Rutschman, Robert ; Doherty, Peter C. ; Murray, Peter J. ; Nienhuis, Arthur W. ; Strom, Ted S. / WASP - mice exhibit defective immune responses to influenza A virus, Streptococcus pneumoniae, and Mycobacterium bovis BCG In: Experimental Hematology. 2005 ; Vol. 33, No. 4. pp. 443-451.
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AU - Mccullers, Jonathan

AU - Lang, Roland

AU - Rutschman, Robert

AU - Doherty, Peter C.

AU - Murray, Peter J.

AU - Nienhuis, Arthur W.

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