West Nile virus infection does not induce PKR activation in rodent cells

Husni Elbahesh, S. V. Scherbik, M. A. Brinton

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

dsRNA-activated protein kinase (PKR) is activated by viral dsRNAs and phosphorylates eIF2a reducing translation of host and viral mRNA. Although infection with a chimeric West Nile virus (WNV) efficiently induced PKR and eIF2a phosphorylation, infections with natural lineage 1 or 2 strains did not. Investigation of the mechanism of suppression showed that among the cellular PKR inhibitor proteins tested, only Nck, known to interact with inactive PKR, colocalized and co-immunoprecipitated with PKR in WNV-infected cells and PKR phosphorylation did not increase in infected Nck1,2-/- cells. Several WNV stem-loop RNAs efficiently activated PKR in vitro but not in infected cells. WNV infection did not interfere with intracellular PKR activation by poly(I:C) and similar virus yields were produced by control and PKR-/- cells. The results indicate that PKR phosphorylation is not actively suppressed in WNV-infected cells but that PKR is not activated by the viral dsRNA in infected cells.

Original languageEnglish (US)
Pages (from-to)51-60
Number of pages10
JournalVirology
Volume421
Issue number1
DOIs
StatePublished - Dec 5 2011

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West Nile virus
Virus Diseases
Rodentia
Phosphorylation
eIF-2 Kinase
Infection
RNA
Viruses
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Virology

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West Nile virus infection does not induce PKR activation in rodent cells. / Elbahesh, Husni; Scherbik, S. V.; Brinton, M. A.

In: Virology, Vol. 421, No. 1, 05.12.2011, p. 51-60.

Research output: Contribution to journalArticle

Elbahesh, Husni ; Scherbik, S. V. ; Brinton, M. A. / West Nile virus infection does not induce PKR activation in rodent cells. In: Virology. 2011 ; Vol. 421, No. 1. pp. 51-60.
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