Whole-body imaging of infection using bioluminescence.

Ying Kong, Yanlin Shi, Mihee Chang, Ali R. Akin, Kevin P. Francis, Ning Zhang, Tamara L. Troy, Hequn Yao, Jianghong Rao, Suat L.G. Cirillo, Jeffrey D. Cirillo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Bioluminescence imaging is a powerful technique to visualize and monitor biological processes in numerous systems. This unit describes two strategies for bioluminescence imaging that can be used to study bacterial infection in mice. One method is to express a luciferase gene in the bacteria; the second method is to use bacteria that express both a luciferase and β-lactamase along with a substrate containing caged luciferin, which is released by β-lactamase hydrolysis and reacts with luciferase to generate light. For both strategies, bioluminescent signals are imaged using an IVIS live animal imaging system (Caliper Life Sciences). The bioluminescence images are analyzed to localize bioluminescent bacteria, quantify signal, and determine the wavelengths of the signals produced. The correlation of bacterial numbers with signal intensity in vivo can be determined, allowing a quantitative measure of bacterial numbers in mice in real time. Methods are described in detail to facilitate successful application of these emerging technologies in nearly any experimental system.

Original languageEnglish (US)
JournalCurrent Protocols in Microbiology
VolumeChapter 2
StatePublished - May 1 2011
Externally publishedYes

Fingerprint

Whole Body Imaging
Luciferases
Bacteria
Infection
Biological Phenomena
Biological Science Disciplines
Bacterial Infections
Hydrolysis
Technology
Light
Genes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Virology

Cite this

Kong, Y., Shi, Y., Chang, M., Akin, A. R., Francis, K. P., Zhang, N., ... Cirillo, J. D. (2011). Whole-body imaging of infection using bioluminescence. Current Protocols in Microbiology, Chapter 2.

Whole-body imaging of infection using bioluminescence. / Kong, Ying; Shi, Yanlin; Chang, Mihee; Akin, Ali R.; Francis, Kevin P.; Zhang, Ning; Troy, Tamara L.; Yao, Hequn; Rao, Jianghong; Cirillo, Suat L.G.; Cirillo, Jeffrey D.

In: Current Protocols in Microbiology, Vol. Chapter 2, 01.05.2011.

Research output: Contribution to journalArticle

Kong, Y, Shi, Y, Chang, M, Akin, AR, Francis, KP, Zhang, N, Troy, TL, Yao, H, Rao, J, Cirillo, SLG & Cirillo, JD 2011, 'Whole-body imaging of infection using bioluminescence.', Current Protocols in Microbiology, vol. Chapter 2.
Kong Y, Shi Y, Chang M, Akin AR, Francis KP, Zhang N et al. Whole-body imaging of infection using bioluminescence. Current Protocols in Microbiology. 2011 May 1;Chapter 2.
Kong, Ying ; Shi, Yanlin ; Chang, Mihee ; Akin, Ali R. ; Francis, Kevin P. ; Zhang, Ning ; Troy, Tamara L. ; Yao, Hequn ; Rao, Jianghong ; Cirillo, Suat L.G. ; Cirillo, Jeffrey D. / Whole-body imaging of infection using bioluminescence. In: Current Protocols in Microbiology. 2011 ; Vol. Chapter 2.
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