Whole spectrum of cytochrome P450 genes and molecular responses to water-accommodated fractions exposure in the marine medaka

Jae Sung Rhee, Bo Mi Kim, Beom Soon Choi, Ik Young Choi, Rudolf S.S. Wu, David Nelson, Jae Seong Lee

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Water-accommodated fractions (WAFs) of crude oil include chemicals that are potent toxicants in fish. Increasing oil pollution thus demands a better understanding of molecular mechanisms for detoxification, metabolism, toxicity, and adaptation of fish. Previous studies with fish show modulation of expression of key genes in relation to stress response against WAF exposure, but there is still a lack of studies on responses of cytochrome P450 (CYP) genes and changes in biotransformation upon WAF exposure. In this study, we used the full spectrum of CYP genes of the marine medaka, Oryzias melastigma, to understand their potential mode of action on WAFs-triggered molecular mechanisms. We also analyzed further CYP-involved detoxification and endogenous steroidogenic metabolism after exposure to different concentrations of WAFs over different time courses in the marine medaka. Also, detoxification- and antioxidant-related enzymes' activities were analyzed with different concentrations of WAFs. As a result, the WAF exposure induced CYP-involved detoxification mechanism but reduced CYP-involved steroidogenic metabolism in the marine medaka. These data suggest that whole CYP profiling can be a way of understanding and uncovering the mode of action particularly with respect to emerging chemicals such as WAF exposure with the new finding that WAFs have dual functions on CYP-involved metabolisms.

Original languageEnglish (US)
Pages (from-to)4804-4812
Number of pages9
JournalEnvironmental Science and Technology
Volume47
Issue number9
DOIs
StatePublished - May 7 2013

Fingerprint

Cytochrome P-450 Enzyme System
cytochrome
Genes
Water
gene
Detoxification
detoxification
Metabolism
metabolism
water
Fish
fish
exposure
oil pollution
Petroleum
Enzyme activity
biotransformation
antioxidant
enzyme activity
crude oil

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Whole spectrum of cytochrome P450 genes and molecular responses to water-accommodated fractions exposure in the marine medaka. / Rhee, Jae Sung; Kim, Bo Mi; Choi, Beom Soon; Choi, Ik Young; Wu, Rudolf S.S.; Nelson, David; Lee, Jae Seong.

In: Environmental Science and Technology, Vol. 47, No. 9, 07.05.2013, p. 4804-4812.

Research output: Contribution to journalArticle

Rhee, Jae Sung ; Kim, Bo Mi ; Choi, Beom Soon ; Choi, Ik Young ; Wu, Rudolf S.S. ; Nelson, David ; Lee, Jae Seong. / Whole spectrum of cytochrome P450 genes and molecular responses to water-accommodated fractions exposure in the marine medaka. In: Environmental Science and Technology. 2013 ; Vol. 47, No. 9. pp. 4804-4812.
@article{16c210dfef574082a32757ee43ef47d5,
title = "Whole spectrum of cytochrome P450 genes and molecular responses to water-accommodated fractions exposure in the marine medaka",
abstract = "Water-accommodated fractions (WAFs) of crude oil include chemicals that are potent toxicants in fish. Increasing oil pollution thus demands a better understanding of molecular mechanisms for detoxification, metabolism, toxicity, and adaptation of fish. Previous studies with fish show modulation of expression of key genes in relation to stress response against WAF exposure, but there is still a lack of studies on responses of cytochrome P450 (CYP) genes and changes in biotransformation upon WAF exposure. In this study, we used the full spectrum of CYP genes of the marine medaka, Oryzias melastigma, to understand their potential mode of action on WAFs-triggered molecular mechanisms. We also analyzed further CYP-involved detoxification and endogenous steroidogenic metabolism after exposure to different concentrations of WAFs over different time courses in the marine medaka. Also, detoxification- and antioxidant-related enzymes' activities were analyzed with different concentrations of WAFs. As a result, the WAF exposure induced CYP-involved detoxification mechanism but reduced CYP-involved steroidogenic metabolism in the marine medaka. These data suggest that whole CYP profiling can be a way of understanding and uncovering the mode of action particularly with respect to emerging chemicals such as WAF exposure with the new finding that WAFs have dual functions on CYP-involved metabolisms.",
author = "Rhee, {Jae Sung} and Kim, {Bo Mi} and Choi, {Beom Soon} and Choi, {Ik Young} and Wu, {Rudolf S.S.} and David Nelson and Lee, {Jae Seong}",
year = "2013",
month = "5",
day = "7",
doi = "10.1021/es400186r",
language = "English (US)",
volume = "47",
pages = "4804--4812",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Whole spectrum of cytochrome P450 genes and molecular responses to water-accommodated fractions exposure in the marine medaka

AU - Rhee, Jae Sung

AU - Kim, Bo Mi

AU - Choi, Beom Soon

AU - Choi, Ik Young

AU - Wu, Rudolf S.S.

AU - Nelson, David

AU - Lee, Jae Seong

PY - 2013/5/7

Y1 - 2013/5/7

N2 - Water-accommodated fractions (WAFs) of crude oil include chemicals that are potent toxicants in fish. Increasing oil pollution thus demands a better understanding of molecular mechanisms for detoxification, metabolism, toxicity, and adaptation of fish. Previous studies with fish show modulation of expression of key genes in relation to stress response against WAF exposure, but there is still a lack of studies on responses of cytochrome P450 (CYP) genes and changes in biotransformation upon WAF exposure. In this study, we used the full spectrum of CYP genes of the marine medaka, Oryzias melastigma, to understand their potential mode of action on WAFs-triggered molecular mechanisms. We also analyzed further CYP-involved detoxification and endogenous steroidogenic metabolism after exposure to different concentrations of WAFs over different time courses in the marine medaka. Also, detoxification- and antioxidant-related enzymes' activities were analyzed with different concentrations of WAFs. As a result, the WAF exposure induced CYP-involved detoxification mechanism but reduced CYP-involved steroidogenic metabolism in the marine medaka. These data suggest that whole CYP profiling can be a way of understanding and uncovering the mode of action particularly with respect to emerging chemicals such as WAF exposure with the new finding that WAFs have dual functions on CYP-involved metabolisms.

AB - Water-accommodated fractions (WAFs) of crude oil include chemicals that are potent toxicants in fish. Increasing oil pollution thus demands a better understanding of molecular mechanisms for detoxification, metabolism, toxicity, and adaptation of fish. Previous studies with fish show modulation of expression of key genes in relation to stress response against WAF exposure, but there is still a lack of studies on responses of cytochrome P450 (CYP) genes and changes in biotransformation upon WAF exposure. In this study, we used the full spectrum of CYP genes of the marine medaka, Oryzias melastigma, to understand their potential mode of action on WAFs-triggered molecular mechanisms. We also analyzed further CYP-involved detoxification and endogenous steroidogenic metabolism after exposure to different concentrations of WAFs over different time courses in the marine medaka. Also, detoxification- and antioxidant-related enzymes' activities were analyzed with different concentrations of WAFs. As a result, the WAF exposure induced CYP-involved detoxification mechanism but reduced CYP-involved steroidogenic metabolism in the marine medaka. These data suggest that whole CYP profiling can be a way of understanding and uncovering the mode of action particularly with respect to emerging chemicals such as WAF exposure with the new finding that WAFs have dual functions on CYP-involved metabolisms.

UR - http://www.scopus.com/inward/record.url?scp=84877617290&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877617290&partnerID=8YFLogxK

U2 - 10.1021/es400186r

DO - 10.1021/es400186r

M3 - Article

VL - 47

SP - 4804

EP - 4812

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 9

ER -