Xenopus oocytes express multiple receptors for LPA-like lipid mediators

Károly Liliom, Kimiko Murakami-Murofushi, Susumu Kobayashi, Hiromu Murofushi, Gabor Tigyi

Research output: Contribution to journalArticle

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Abstract

In Xenopus laevis oocytes, both lysophosphatidic acid (LPA) and a cyclic phosphate-containing analogue 1-acyl-sn-glycero-2,3-cyclic phosphate (cLPA) isolated from Physarum polycephalum activate oscillatory Cl currents. cLPA elicited oscillatory currents only when applied extracellularly and, similarly to LPA, evoked homologous desensitization. cLPA applied to oocytes previously desensitized by LPA failed to elicit a current, indicating that LPA completely desensitized the cLPA receptors. In contrast, when oocytes were desensitized by cLPA, LPA still evoked large currents. The lack of heterologous desensitization between cLPA and LPA indicates that the former acts on a distinct receptor subpopulation(s), which is also activated by LPA. The alkyl-ether analogue 1-hexadecyl-2-lyso-sn-glycero-3-phosphate (16:0-GP) and dioleoyl-phosphatidic acid (18:1-PA) showed heterologous desensitization patterns similar to that of LPA with regard to cLPA. Complete heterologous desensitization was obtained between LPA and 16:0-GP or 18:1-PA. These observations demonstrate the simultaneous expression of at least two different types of receptors for LPA-like lipid mediators on Xenopus oocytes and that these receptors show different pharmacological properties in their selectivity to cLPA.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume270
Issue number3 39-3
StatePublished - Mar 1 1996

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Lysophosphatidic Acid Receptors
Xenopus
Oocytes
Phosphates
Lipids
Physarum polycephalum
lysophosphatidic acid
Phosphatidic Acids
Xenopus laevis
Ether

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

Liliom, K., Murakami-Murofushi, K., Kobayashi, S., Murofushi, H., & Tigyi, G. (1996). Xenopus oocytes express multiple receptors for LPA-like lipid mediators. American Journal of Physiology - Cell Physiology, 270(3 39-3).

Xenopus oocytes express multiple receptors for LPA-like lipid mediators. / Liliom, Károly; Murakami-Murofushi, Kimiko; Kobayashi, Susumu; Murofushi, Hiromu; Tigyi, Gabor.

In: American Journal of Physiology - Cell Physiology, Vol. 270, No. 3 39-3, 01.03.1996.

Research output: Contribution to journalArticle

Liliom, K, Murakami-Murofushi, K, Kobayashi, S, Murofushi, H & Tigyi, G 1996, 'Xenopus oocytes express multiple receptors for LPA-like lipid mediators', American Journal of Physiology - Cell Physiology, vol. 270, no. 3 39-3.
Liliom, Károly ; Murakami-Murofushi, Kimiko ; Kobayashi, Susumu ; Murofushi, Hiromu ; Tigyi, Gabor. / Xenopus oocytes express multiple receptors for LPA-like lipid mediators. In: American Journal of Physiology - Cell Physiology. 1996 ; Vol. 270, No. 3 39-3.
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