Yeast TFIID serves as a coactivator for Rap1p by direct protein-protein interaction

Krassimira A. Garbett, Manish Tripathi, Belgin Cencki, Justin H. Layer, P. Anthony Weil

Research output: Contribution to journalArticle

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Abstract

In vivo studies have previously shown that Saccharomyces cerevisiae ribosomal protein (RP) gene expression is controlled by the transcription factor repressor activator protein 1 (Rap1p) in a TFIID-dependent fashion. Here we have tested the hypothesis that yeast TFIID serves as a coactivator for RP gene transcription by directly interacting with Rap1p. We have found that purified recombinant Rap1p specifically interacts with purified TFIID in pull-down assays, and we have mapped the domains of Rap1p and subunits of TFIID responsible. In vitro transcription of a UASRAP1 enhancer-driven reporter gene requires both Rap1p and TFIID and is independent of the Fhl1p-Ifh1p coregulator. UASRAP1 enhancer-driven transactivation in extracts depleted of both Rap1p and TFIID is efficiently rescued by addition of physiological amounts of these two purified factors but not TATA-binding protein. We conclude that Rap1p and TFIID directly interact and that this interaction contributes importantly to RP gene transcription.

Original languageEnglish (US)
Pages (from-to)297-311
Number of pages15
JournalMolecular and cellular biology
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2007

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Transcription Factor TFIID
Yeasts
Ribosomal Proteins
Proteins
TATA-Box Binding Protein
Repressor Proteins
Saccharomyces cerevisiae Proteins
Transcription Factor AP-1
Reporter Genes
Transcriptional Activation
Genes
Transcription Factors
Gene Expression

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Yeast TFIID serves as a coactivator for Rap1p by direct protein-protein interaction. / Garbett, Krassimira A.; Tripathi, Manish; Cencki, Belgin; Layer, Justin H.; Weil, P. Anthony.

In: Molecular and cellular biology, Vol. 27, No. 1, 01.01.2007, p. 297-311.

Research output: Contribution to journalArticle

Garbett, Krassimira A. ; Tripathi, Manish ; Cencki, Belgin ; Layer, Justin H. ; Weil, P. Anthony. / Yeast TFIID serves as a coactivator for Rap1p by direct protein-protein interaction. In: Molecular and cellular biology. 2007 ; Vol. 27, No. 1. pp. 297-311.
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